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B8522

Sigma-Aldrich

Brilliant Blue G solution

Concentrate

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About This Item

Número CAS:
Número MDL:
Código UNSPSC:
12171500
ID de substância PubChem:
NACRES:
NA.47

forma

liquid

cor

dark blue

adequação

suitable for (Suitable for for staining protein in polyacrylamide and agarose gels)

aplicação(ões)

diagnostic assay manufacturing
hematology
histology

temperatura de armazenamento

2-8°C

cadeia de caracteres SMILES

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3C)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=CC(\C=C5C)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI

1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30H,6-8,31-32H2,1-5H3,(H2,51,52,53,54,55,56);/q;+1/p-1

chave InChI

RWVGQQGBQSJDQV-UHFFFAOYSA-M

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Descrição geral

Brilliant Blue G is a methanol based stain which is especially designed for staining protein in polyacrylamide gels, but will also stain protein in agarose gels (IEF). The stain contains methanol and acetic acid so gels do not require fixing prior to staining. Water is added directly to the bottle to produce 1 liter of ready-to-use staining solution.

Aplicação

Brilliant Blue G solution has been used for protein band detection after SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). It is also used for the protein staining of cells on microscopic slide.

Quantidade

One bottle equals one liter working solution. 300 ml of brilliant blue G solution concentrate is supplied in the bottle.

Reconstituição

After dilution with deionized water to a final volume of one liter, solution will contain 0.1% (w/v) Brilliant Blue G, 25% (v/v) methanol and 5% (v/v) acetic acid.

Nota de análise

This product has been tested for suitability on SDS-PAGE.

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Preços

Ponto de fulgor (°F)

49.5 °F - closed cup

Ponto de fulgor (°C)

9.7 °C - closed cup

Equipamento de proteção individual

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


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