11815016001
Roche
Anti-HA Affinity Matrix
from rat IgG1
Sinônimo(s):
affinity matrix, anti-ha
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Código UNSPSC:
12352203
Preço e disponibilidade não estão disponíveis no momento.
Produtos recomendados
fonte biológica
rat
Nível de qualidade
conjugado
agarose conjugate
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
3F10, monoclonal
Formulário
slurry
embalagem
pkg of 1 mL (settled resin volume)
fabricante/nome comercial
Roche
Isotipo
IgG1
sequência de epítopo
YPYDVPDYA
capacidade
2-8 nmol/mL binding capacity
temperatura de armazenamento
2-8°C
Categorias relacionadas
Descrição geral
Anti-HA High Affinity antibody (clone 3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins that are derived from mammalian, bacterial, and yeast expression vectors, regardless of its position (N-terminal, C-terminal or internal)
Especificidade
Anti-HA High Affinity antibody (clone 3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins that are derived from mammalian, bacterial, and yeast expression vectors, regardless of its position (N-terminal, C-terminal or internal).
Imunogênio
Amino acids 98-106 from the human influenza virus hemagglutinin protein
Aplicação
Following immunoprecipitation or purification, the tagged protein of interest may be analyzed by:
- Western blotting using the Anti-HA antibody
- Silver staining (or similar protein stain)
- In coimmunoprecipitation.[1]
Características e benefícios
- Achieve enhanced specificity and reduced cross-reactivity of Anti-HA High Affinity.
- Use nondenaturing conditions for elution of your HA-tagged protein of choice.
- Purify even rarely expressed HA-tagged proteins.
- Highly specific to YPYDVPDYA.
- Suitable for purification of proteins containing HA-epitop as N-terminal, C-terminal or internal fusion.
- Applicable with crued cell extracts from mammalian, bacterial and yeast expression systems.
Qualidade
Each lot of Anti-HA Affinity Matrix is tested for its ability to purify a HA-tagged protein expressed in stably transfected cells from a crude mammalian extract. The immunoprecipitate is analyzed by western blot using Anti-HA, High Affinity antibody.
forma física
1 ml settled resin of Anti-HA Affinity Matrix in PBS containing 0.09% sodium azide (w/v); 2 ml suspension equals to 1 ml bed volume. One plastic column with top and bottom caps is included.
Nota de análise
Yield of 9 nmol purified protein/ml affinity matrix was determined using a whole-cell bacterial extract containing expressed HA-tagged bacterial alkaline phosphatase.
Outras notas
For life science research only. Not for use in diagnostic procedures.
Não está encontrando o produto certo?
Experimente o nosso Ferramenta de seleção de produtos.
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
does not flash
Ponto de fulgor (°C)
does not flash
Escolha uma das versões mais recentes:
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Os clientes também visualizaram
Abdirahman Abdi et al.
Cellular and molecular life sciences : CMLS, 67(19), 3355-3369 (2010-06-29)
Over the last decade, several protein kinases inhibitors have reached the market for cancer chemotherapy. The kinomes of pathogens represent potentially attractive targets in infectious diseases. The functions of the majority of protein kinases of Plasmodium falciparum, the parasitic protist
Geetha Hewawasam et al.
Molecular cell, 40(3), 444-454 (2010-11-13)
Cse4 is a variant of histone H3 that is incorporated into a single nucleosome at each centromere in budding yeast. We have discovered an E3 ubiquitin ligase, called Psh1, which controls the cellular level of Cse4 via ubiquitylation and proteolysis.
Stephen C Kales et al.
PloS one, 9(1), e87116-e87116 (2014-01-28)
The Cbl proteins (Cbl, Cbl-b, and Cbl-c) are a highly conserved family of RING finger ubiquitin ligases (E3s) that function as negative regulators of tyrosine kinases in a wide variety of signal transduction pathways. In this study, we identify a
Jinyan Cheng et al.
Journal of virology, 94(7) (2020-01-17)
Stress granules (SGs) are formed in the cytoplasm under environmental stress, including viral infection. Human enterovirus D68 (EV-D68) is a highly pathogenic virus which can cause serious respiratory and neurological diseases. At present, there is no effective drug or vaccine
Wim Schepers et al.
The Journal of biological chemistry, 287(53), 44130-44142 (2012-11-17)
The readdition of an essential nutrient to starved, fermenting cells of the yeast Saccharomyces cerevisiae triggers rapid activation of the protein kinase A (PKA) pathway. Trehalase is activated 5-10-fold within minutes and has been used as a convenient reporter for
Protocolos
Anti-HA Affinity Matrix Protocol & Troubleshooting
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica
