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Documentos Principais

PHCC360KIT

Millipore

Contador de células automatizado portátil Scepter 3.0

Scepter 3.0 Handheld Automated Cell Counter

Rapid cell counts in a handheld easy to use format, includes pkg of 60 μm Scepter 3.0 Cell Counter Sensors

Sinônimo(s):

Cell counting instrument

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About This Item

Código UNSPSC:
41131501
NACRES:
NC.56
Preço e disponibilidade não estão disponíveis no momento.

fabricante/nome comercial

Scepter

técnica(s)

cell counting: suitable

tamanho de partícula

6-36 μm

compatibilidade

for use with cells sized 4.0–25.0 μm

Condições de expedição

ambient

Descrição geral

O contador de células automatizado portátil Scepter 3.0 oferece contagens rápidas de células em um formato portátil e fácil de usar. Destinado apenas para uso em pesquisa, o Scepter 3.0 foi testado com tipos de células representativos daqueles em uso atualmente. Após preparar uma diluição da cultura celular de interesse, as instruções na tela orientam o usuário pelo processo de amostragem. Em menos de 30 segundos após o contador de células de cetro aspirar uma amostra para o sensor do contador de células de cetro, a tela do contador de células de cetro exibe:

  • Concentração de células
  • Tamanho celular médio
  • Volume celular médio
  • Histograma da distribuição de tamanhos ou volumes
O dispositivo armazena até 999 contagens, que podem ser baixadas para o computador do usuário e carregadas para posterior manipulação.

O contador de células Scepter 3.0 é compatível com os sensores de contador de células Scepter 3.0 de 40 uM e 60 uM. Esse kit (PHCC360KIT) inclui um pacote gratuito de 50 sensores de 60 uM.

Componentes

  • 1 Scepter 3.0 Contador de células portátil automatizado
  • 1 frasco de contas de teste
  • 1 estação de carregamento
  • 50 sensores (60μM)

Outras notas

O contador de células Scepter 3.0 não é compatível com sensores da geração anterior; compatível somente com os sensores de contador de células Scepter 3.0 mostrados nos Produtos relacionados.

Código de classe de armazenamento

10-13 - German Storage Class 10 to 13

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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Artigos

Data for validated human cell lines using the Millicell® DCI Digital Cell Imager for confluency determination and estimated cell counts.

The Scepter™ 3.0 Cell Counter, when used in conjunction with a 40 μm aperture sensor, can accurately count and precisely size a wide range of cell types and particles. This application note demonstrates two examples of experiments using the instrument’s sensitive size-discriminating capacity for rapid qualitative assessment of individual cell populations.

Find answers to frequently asked questions about the Millicell® ERS 3.0, from probe depth adjustment to instrument maintenance and troubleshooting.

Protocolos

Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.

Conteúdo relacionado

Simplifique a captura e análise de dados da resistência elétrica transepitelial ou transendotelial (TEER) com melhorias fáceis de usar, incluindo uma interface intuitiva com tela digital, sonda permanente no poço e registro automático de dados.

Monitor your cell cultures with ease with the Millicell® DCI Digital Cell Imager. From confluency to cell count and morphology, the Millicell® DCI automates cell culture measurements for quicker, more consistent culturing.

Scepter™ Cell Counter is a portable device which brings consistency in cell counting right to the culture hood in less than 30 seconds.

O contador de células Scepter™ é um dispositivo portátil que viabiliza uma contagem celular consistente diretamente na câmara de cultura em menos de 30 segundos.

Ver tudo

Questions

1–3 of 3 Questions  
  1. What is the maximum size of cell clumps that can pass through the Scepter 3.0? Also, what size screen is used to break up the cell clump aggregates in both Scepter 2.0 and 3.0?

    1 answer
    1. The maximum size of cell clumps that can pass through the Scepter 3.0 is determined by the aperture limits, which are 40um and 60um for sensors 40 and 60, respectively. Unlike the Scepter 2.0, the Scepter 3.0 does not use a screen to break up cell clump aggregates; instead, it employs a set of pillars to achieve this function. In contrast, the Scepter 2.0 utilizes a 35um Nylon filter to break up cell clump aggregates.

      Helpful?

  2. Is this appropriate for size 15-23 μm cell? Does the Kit include also the charger? How many 60/40 μm sensors are included Which sensors can be used with this instrument? Which sensors are appropriate for cells size 15-23 μm and what is their price?

    1 answer
    1. Please see the answers to the following questions below:

      1. Is this appropriate for size 15-23 μm cells?
      Yes, the Scepter 3.0 is suitable. As listed on the product detail page in the DESCRIPTION section for use with cells sized 4.0–25.0 μm.

      2. Does the Kit include the charger?
      This product does include 60μM sensors and a charging station.

      3. How many 60/40 μm sensors are included
      As mentioned on the product detail page in the DESCRIPTION section under 'Components', it includes 50 Sensors.

      4. Which sensors can be used with this instrument?
      This product is only compatible with Scepter 3.0 Cell Counter Sensors shown in the Related Products on this page. Please see the link below to review additional information regarding the Scepter 3.0:
      https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/cell-counting-and-health-analysis/scepter-cell-counter

      5. Which sensors are appropriate for cells size 15-23 μm and what is their price?
      See question 1, this product is suitable for the cell size of 15-23 μm.

      Regarding pricing, Please navigate to the link https://www.sigmaaldrich.com/support/customer-support, and click on "Products Pricing & Availability" under the Products Section with all the required information so that a member of the Customer Service team can reach out to assist further. Thank you.

      Helpful?

  3. Does it require Trypan blue or another dye? Does it distinguish between live and dead cells?

    1 answer
    1. The Scepter is a particle counter, but it will not differentiate live from dead cells. The device can be used to gauge cell health on the basis of cell size. For example, in cell types where apoptotic cells are smaller in size compared to non-apoptotic cells. This shift in size can be quantified using the device option "Edit Histogram" to either gate around this population or exclude the population from the count.

      Please see the following manual below for more information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/799/484/00110230w-rev1020-ms.pdf

      Helpful?

Reviews

Active Filters

  1. R. Moldova
    • Review 1
    • Votes 4
    3 out of 5 stars.

    Suggestion

    I could have been more careful when ordering the Scepter 3.0 components (Handheld Automated Cell Counter and Cell sensors), because without experience I ordered sensors not suitable for this type of reader. That is, instead of PHCC360500
    Cell Counter Sensors I ordered PHCC60500.
    No one raised any questions. Regrettable. I will have to incur additional expenses.
    I really hope to fix this mistake to be able to apply this device in practice.

    Helpful?

    1. Response from MilliporeSigma:

      Thank you for reaching out and sharing your review with us. We strive to provide the information customers need to make a confident purchase. Your feedback is extremely valuable in helping us to update the product information for this product to clarify which sensors are compatible with the Scepter 3.0.

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