MAB3418X
Anti-MAP2 Antibody, Alexa Fluor™ 488 conjugated
clone AP20, Chemicon®, from mouse
Sinônimo(s):
Anti-MAP-2, Anti-MAP2A, Anti-MAP2B, Anti-MAP2C
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Preço e disponibilidade não estão disponíveis no momento.
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
conjugado
ALEXA FLUOR™ 488
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
AP20, monoclonal
reatividade de espécies
rat, human, chicken, mouse, bovine
fabricante/nome comercial
Chemicon®
técnica(s)
immunohistochemistry: suitable
Isotipo
IgG1
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
chicken ... Map2(424001)
human ... MAP2(4133)
mouse ... Map2(17756) , Map2(281294)
rat ... Map2(25595)
Especificidade
Recognizes MAP-2 (microtubule associated protein-2). MAP-2 is one of several high molecular weight proteins that play an important role in brain microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles (1).
MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2 (2,3). MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds (4,5).
SDS-PAGE Profiles: In SDS-PAGE MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 300 kD. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier).
MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2 (2,3). MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds (4,5).
SDS-PAGE Profiles: In SDS-PAGE MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 300 kD. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier).
Imunogênio
Bovine brain microtubule protein.
Aplicação
Immunohistochemistry
Optimal working dilutions must be determined by end user.
APPLICATION NOTES:
Immunohistochemistry: Anti-MAP-2 can be used to stain tissue (brain or spinal cord) fixed with paraformaldehyde.
Optimal working dilutions must be determined by end user.
APPLICATION NOTES:
Immunohistochemistry: Anti-MAP-2 can be used to stain tissue (brain or spinal cord) fixed with paraformaldehyde.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
Neurofilament & Neuron Metabolism
This Anti-MAP2 Antibody, Alexa Fluor 488 conjugated is validated for use in IH for the detection of MAP2.
forma física
Protein A purified
Purified immunoglobulin conjugated to Alexa Fluor™ 488. Liquid in Phosphate buffer with 15 mg/mL BSA as a stabilizer and 0.1% sodium azide.
Armazenamento e estabilidade
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Nota de análise
Control
Brain, Neuronal culture, Human glioblastoma T98G cells
Brain, Neuronal culture, Human glioblastoma T98G cells
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informações legais
ALEXA FLUOR is a trademark of Life Technologies
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Alexa Fluor™ is a registered trademark of Molecular Probes, Inc.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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