69050
Anti-Cre Antibody
Novagen®
Sinônimo(s):
Cre Recombinase Antibody
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.43
Produtos recomendados
forma do anticorpo
purified antibody
Nível de qualidade
tipo de produto de anticorpo
primary antibodies
fabricante/nome comercial
Novagen®
condição de armazenamento
OK to freeze
avoid repeated freeze/thaw cycles
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
modificação pós-traducional do alvo
unmodified
Descrição geral
Recombinase cre (UniProt: P06956; also known as Cre) is encoded by the cre gene (Gene ID: 2777477) in Escherichia phage P1 (Bacteriophage P1). Cre (Causes recombination with Cyclization recombinase), a 38 kDa DNA recombinase of the ′phage′ integrase family, is derived from the P1 bacteriophage. It catalyzes site-specific recombination and is highly specific for a 34-bp DNA sequence motif called loxP (locus of X-over P1) found in P1 DNA. The loxP site is composed of a variable asymmetric 8-bp sequence in between two sets of 13-bp palindromic sequences (ATAACTTCGTATA-NNNTANNN-TATACGAAGTTAT). Two cre recombinase proteins, each bind to one of the two 13-bp palindromic sequences at a loxP site, form a dimer. This dimer then binds to a second dimer on another loxP site to form a tetramer. The two sites joined by the tetramer are parallel in orientation and the double stranded DNA is cut at both loxP sites. The strands are then rejoined with DNA ligase. Because the Cre gene and loxP sites are not native to most species, they are often introduced into target cells or tissues and employed as a means to precisely control the expression of genes both in cultured cells in vitro and in animals in vivo.
Specific, sensitive detection of Cre recombinase in cells and cell lysates
Supplied as purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine, 0.15 M NaCl, pH 7.4 with 0.05% sodium azide and 50% glycerol.
Evaluated by Western Blotting with Recombinant Cre-His.
Western Blotting Analysis: Representative lot data.Recombinant Cre-His loaded at 20 ng/lane (Lane 1) and 10 ng/lane (Lane 2) was probed with Cat. No. 69050-3, Anti-Cre (1:10,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Cre (~39 kDa).
Evaluated by Western Blotting with Recombinant Cre-His.
Western Blotting Analysis: Representative lot data.Recombinant Cre-His loaded at 20 ng/lane (Lane 1) and 10 ng/lane (Lane 2) was probed with Cat. No. 69050-3, Anti-Cre (1:10,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Cre (~39 kDa).
Advertência
Toxicity: Standard Handling (A)
Informações legais
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
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