454578

Anti-Pan-NMDA NR1 Mouse mAb (R1JHL)

lyophilized, clone R1JHL, Calbiochem^®

• Código UNSPSC: 12352203
• NACRES: NA.43

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: culture supernatant
• tipo de produto de anticorpo: primary antibodies
• clone: R1JHL, monoclonal
• Formulário: lyophilized
• não contém: preservative
• reatividade de espécies: rat
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• Isotipo: IgG
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C
• modificação pós-traducional do alvo: unmodified

Descrição

Descrição geral

Mouse monoclonal antibody lyophilized from cell culture supernatant. Recognizes the ~115 kDa subunit of the NMDAR (N-methyl-D-aspartate receptor) protein.

Recognizes the ~115 kDa NMDA receptor NR1 subunit in rat cortex tissue extracts.

This Anti-Pan-NMDA NR1 Mouse mAb (R1JHL) is validated for use in Immunoblotting for the detection of Pan-NMDA NR1.

Imunogênio

Rat

a recombinant fusion protein consisting of amino acids 1-564 of rat NMDA NR1

Aplicação

Immunoblotting (1:300, colorimetric)

Immunoprecipitation (3 µg/200 µg lysate)

Advertência

Toxicity: Standard Handling (A)

forma física

Lyophilized from cell culture supernatant.

Reconstituição

Reconstitute in 50 µl PBS (137 mM NaCl, 7.5 mM Na₂HPO₄, 2.7 mM KCl, 1.5 mM KH₂P0₄, pH 7.4)Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

Nota de análise

Positive Control
Rat cortex tissue extracts

Outras notas

Does not cross-react with other NMDAR subunits. For immunoblotting, dilute in TBST containing 5% nonfat milk and 0.05% sodium azide. Antibody may be used up to 5 times in immunoblotting experiments without significant loss in signal strength. Store diluted antibody at 4°C between uses. Variables associated with assay conditions will dictate the proper working dilution.

Laurie, D.J., and Seeburg, P.H. 1994. J. Neurosci.14, 3180
Ishii, T., et al. 1993. J. Biol. Chem.268, 2836.
Monyer, H., et al. 1992. Science256, 1217.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable