331569

Ethidium Bromide Adsorber

Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels.

• Sinônimo(s): Ethidium Bromide Adsorber
• Número CAS: 69011-20-7
• Código UNSPSC: 12352200
• NACRES: NA.25

Propriedades

• Nível de qualidade: 200
• forma: suspension
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• cor: gray-brown
• Condições de expedição: ambient
• temperatura de armazenamento: 15-25°C
• InChI: 1S/C10H12.C10H10.C8H8/c2*1-3-9-7-5-6-8-10(9)4-2;1-2-8-6-4-3-5-7-8/h3,5-8H,1,4H2,2H3;3-8H,1-2H2;2-7H,1H2
• chave InChI: NWUYHJFMYQTDRP-UHFFFAOYSA-N

Descrição

Descrição geral

Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels. Typical concentrations in these solutions are 0.5 - 10 mg/L. EtBr is bound and concentrated by the adsorber column, allowing removal of the filtrate. Each column normally binds at least 300 mg of ethidium bromide from TAE or TBE buffers. Residual capacity is easily visualized because the EtBr appears as a dark red to black band on the column. As long as this band has not reached the bottom of the column bed, the concentration of dye is still below the column capacity.

Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels. Typical concentrations in these solutions are 0.5-10 mg/l. EtBr is bound and concentrated by the adsorber column, allowing removal of the filtrate. Each column normally binds at least 300 mg of ethidium bromide from TAE or TBE buffers. Residual capacity is easily visualized because the EtBr appears as a dark red to black band on the column. As long as this band has not reached the bottom of the column bed, the concentration of dye is still below the column capacity.

Ações bioquímicas/fisiológicas

Cell permeable: no

Primary Target
Removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers

Product does not compete with ATP.

Reversible: no

Advertência

Toxicity: Irritant (B)

Reconstituição

When the adsorber column is not in use, both ends should be closed off. The column should not be allowed to dry out, as air bubbles may form in the column, hindering flow rate.

Outras notas

MacGregor, J.T., and Johnson, I.J. 1977. Mutat. Res.48, 103.
Waring, M. 1975. In: Antibiotics, Corcoran, J.W., and Hahn, F.E., Editors, Vol. 3, 141, Cold Spring Harbor, NY.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H319
• Declarações de precaução: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Classificações de perigo: Eye Irrit. 2
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable