219364
Cathepsin B, Human Liver
Cathepsin B, Human Liver, CAS 9047-22-7, is a purified native cathepsin B from human liver, purified by affinity chromatography. Upregulated in many types of tumors.
Sinônimo(s):
Cathepsin B, Human Liver
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About This Item
Produtos recomendados
fonte biológica
human liver
Nível de qualidade
Ensaio
>95% (SDS-PAGE)
forma
liquid
atividade específica
≥50 units/mg protein
purificado por
affinity chromatography
fabricante/nome comercial
Calbiochem®
condição de armazenamento
OK to freeze
avoid repeated freeze/thaw cycles
técnica(s)
activity assay: suitable
faixa de pH
3.5—6.0
adequação
suitable for molecular biology
aplicação(ões)
life science and biopharma
Condições de expedição
wet ice
temperatura de armazenamento
−70°C
Informações sobre genes
human ... CTSB(1508)
Descrição geral
Research area: Cell Signaling
Cathepsin B, Human Liver, is a native cathepsin B from human liver, purified by affinity chromatography. Cathepsin B is a lysosomal protease with endo/exopeptidase and a carboxydipeptidase activity. It is also known as cysteine cathepsin and belongs to the C1 family of papain-like enzymes. Cathepsin B like H, L, C, X, F, O and V is ubiquitously expressed in human tissues.
Optimal pH: 3.5-6.0.
Cathepsin B, Human Liver, is a native cathepsin B from human liver, purified by affinity chromatography. Cathepsin B is a lysosomal protease with endo/exopeptidase and a carboxydipeptidase activity. It is also known as cysteine cathepsin and belongs to the C1 family of papain-like enzymes. Cathepsin B like H, L, C, X, F, O and V is ubiquitously expressed in human tissues.
Optimal pH: 3.5-6.0.
Aplicação
Cathepsin B, Human Liver has been used to assess its linker cleavage or proteolytic activity using an LC/MS MRM-based quantitation method.
Ações bioquímicas/fisiológicas
Cathepsin B facilitates non-specific, bulk proteolysis within the acidic conditions of the endosomal/lysosomal compartment. Here, they break down both intracellular and extracellular proteins. Upregulation and secretion of cathepsin B have been shown to occur in many types of tumors and to correlate positively with their invasive and metastatic capabilities. Cathepsin B is shown to facilitate tumor invasion by dissolving extracellular barriers.
Embalagem
Please refer to vial label for lot-specific concentration.
Advertência
Toxicity: Standard Handling (A)
Definição da unidade
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-RR-βNA per min at 40°C, pH 6.0 in the presence of DTT.
forma física
In 50 mM sodium acetate buffer, 1 mM EDTA, pH 5.0.
Nota de preparo
Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Reconstituição
Following initial thaw, aliquot and freeze (-70°C).
Outras notas
Kostoulas, G., et al. 1999. FEBS Lett. 455, 286.
Berquim, I.M., and Sloane, B.F. 1996. Adv. Exp. Med. Biol.389, 281.
Keppler, D., et al. 1994. Biochim. Biophys. Acta.1226, 117.
Musil, D., et al. 1991. EMBO J.10, 2321.
Sloane B.F. 1990. Semin. Cancer Biol.1, 137.
Berquim, I.M., and Sloane, B.F. 1996. Adv. Exp. Med. Biol.389, 281.
Keppler, D., et al. 1994. Biochim. Biophys. Acta.1226, 117.
Musil, D., et al. 1991. EMBO J.10, 2321.
Sloane B.F. 1990. Semin. Cancer Biol.1, 137.
Informações legais
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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