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123072

Sigma-Aldrich

Luminol

97%

Sinônimo(s):

3-Aminophthalhydrazide, 5-Amino-2,3-dihydro-1,4-phthalazinedione

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About This Item

Fórmula empírica (Notação de Hill):
C8H7N3O2
Número CAS:
521-31-3
Peso molecular:
177.16
Beilstein:
383929
Número CE:
208-309-4
Número MDL:
MFCD00006890
Código UNSPSC:
12352103
ID de substância PubChem:
24847519
NACRES:
NA.23

Nível de qualidade

200

Ensaio

97%

pf

>300 °C (lit.)

cadeia de caracteres SMILES

Nc1cccc2C(=O)NNC(=O)c12

InChI

1S/C8H7N3O2/c9-5-3-1-2-4-6(5)8(13)11-10-7(4)12/h1-3H,9H2,(H,10,12)(H,11,13)

chave InChI

HWYHZTIRURJOHG-UHFFFAOYSA-N

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Descrição geral

Luminol is a very efficient and commonly used chemiluminiscent compound in condensed phase. When basic solutions of luminol containing oxygen are treated with an oxidizing agent, light is produced. Oxidising agents capable of initiating this particular reaction are Nitrogen dioxide, PAN, hydrogen peroxide, ozone and other atmospheric oxidants.

Aplicação

  • As a chemiluminiscent substance. Used to measure opsonic and phagocytic function.
  • As a biological sensor. Used to detect the polymorphonuclear leukocytes (PMNL) response in a patient with a myeloperoxidase (MPO) deficiency.
  • Used as a forensic test for blood.
Luminol has been used in a protocol to study the immune-related responses in Arabidopsis thaliana via luminol/peroxidase-based assay.

Características e benefícios

  • Very effective chemiluminiscent compound.
  • Can detect sub-part per billion levels of metal ions with simple and inexpensive instrumentation.
Used for chemiluminescence analysis of, for example, metal cations, blood, and glucococorticoids.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Gloves

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
SHBR5033
SHBR5032
SHBR5034
SHBR5035
SHBR1903

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4-Iodophenol 99%

Sigma-Aldrich

I10201

4-Iodophenol

N-(4-Aminobutyl)-N-ethylisoluminol ≥90%

Sigma-Aldrich

A0156

N-(4-Aminobutyl)-N-ethylisoluminol

L-012 sodium salt ≥98% (HPLC)

Sigma-Aldrich

SML2236

L-012 sodium salt

4-Morpholinopyridine 97%

Sigma-Aldrich

480657

4-Morpholinopyridine

Fast gas chromatography with luminol chemiluminescence detection for the simultaneous determination of nitrogen dioxide and peroxyacetyl nitrate in the atmosphere
Marley, Nancy A., et al.
The Review of Scientific Instruments, 75, 4595-4605 (2004)
Luminol-based assay for detection of immunity elicitor-induced hydrogen peroxide production in Arabidopsis thaliana leaves.
Bisceglia NG, et al.
Bio-protocol, 5, e1685-e1685 (2015)
C S Easmon et al.
Immunology, 41(1), 67-74 (1980-09-01)
Luminol-dependent chemiluminescence produced by peripheral blood phagocytic cells was measured using a light detecting instrument, the Luminometer 1250 (LKB Wallac). Although less sensitive than liquid scintillation counters, the Luminometer can measure chemiluminescence effectively and detect experimentally induced and clinical variations
T I Quickenden et al.
Luminescence : the journal of biological and chemical luminescence, 16(4), 295-298 (2001-08-21)
A wide range of domestic and industrial substances that might be mistaken for haemoglobin in the forensic luminol test for blood were examined. The substances studied were in the categories of vegetable or fruit pulps and juices; domestic and commercial
C Dahlgren et al.
Infection and immunity, 39(2), 736-741 (1983-02-01)
When polymorphonuclear leukocytes (PMNL) and soluble or particulate matter interact, the cells produce chemiluminescence, linked to activation of the oxidative metabolism of the cells. PMNL isolated from a patient with a myeloperoxidase deficiency were found to produce almost no luminol-dependent
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