The guard column kit for Product No. 565325-U is 565371-U. This kit includes one cartridge, stand-alone holder, a piece of tubing, 2 nuts and 2 ferrules. A set of two replacement cartridges is Product No. 565370-U.
565325-U
Ascentis® RP-Amide (5 µm) HPLC Columns
L × I.D. 25 cm × 4.6 mm, HPLC Column
Synonym(e):
RP-Amide Reversed-Phase Chromatography Column
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About This Item
UNSPSC-Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
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Produktbezeichnung
Ascentis® RP-Amid HPLC-Säule, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Materialien
stainless steel column
Qualitätsniveau
Agentur
suitable for USP L60
Produktlinie
Ascentis®
Leistungsmerkmale
endcapped
Hersteller/Markenname
Ascentis®
Verpackung
1 ea of
Kennzeichnungsgrad
19.5% Carbon loading
Parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
Methode(n)
HPLC: suitable
LC/MS: suitable
L × ID
25 cm × 4.6 mm
Oberflächenbereich
450 m2/g
Oberflächendeckung
2.7 μmol/m2
Verunreinigungen
<5 ppm metals
Matrix
fully porous particle
silica gel high purity, spherical
Aktive Matrixgruppe
amide, alkyl phase
Partikelgröße
5 μm
Porengröße
100 Å
pH Betriebsbereich
2-8
Anwendung(en)
environmental
food and beverages
Trenntechnik
reversed phase
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Verwandte Kategorien
Allgemeine Beschreibung
Die Ascentis RP-Amide ist eine neue Generation äußerst gering blutender Phasen mit eingebetteter polarer Gruppe (EPG), die orthogonale Selektivität und eine hohe Auflösung bei der HPLC- oder LC-MS-Analyse polarer Verbindungen liefert. Die Ascentis RP-Amide ist die erste Wahl von HPLC-Phasen mit eingebeteten polaren Gruppen.
Anwendung
- A validated UHPLC method for the determination of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts using an RP-Amide fused-core column.: This study presents a validated Ultra-High-Performance Liquid Chromatography (UHPLC) method employing an RP-Amide fused-core column for the quantitative determination of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. The method demonstrated excellent precision, accuracy, and sensitivity, making it a valuable tool for the analysis of these bioactive compounds in complex matrices. The research highlights the utility of the Ascentis® RP-Amide HPLC Column in enhancing the resolution and efficiency of chromatographic separations in analytical chemistry applications (Fibigr et al., 2018).
Leistungsmerkmale und Vorteile
- Ausgezeichnete Retention und Peak-Form für polare Verbindungen
- Kompatibel mit 100% wässrigen Lösungen
- Äußerst geringes Bluten, LC-MS-kompatibel
- Einzigartige Selektivität
Rechtliche Hinweise
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Vorsäulenkartusche
Ähnliches Produkt
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Rihana Parveen Shaik et al.
Journal of pharmaceutical analysis, 3(6), 489-499 (2013-12-01)
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used for simultaneous quantification of tolterodine and its metabolite 5-hydroxy methyl tolterodine in rat plasma. Tolterodine-d6 and 5-hydroxy methyl tolterodine-d14 were used as internal standards (IS). Chromatographic separation was performed on Ascentis Express
Jakub Fibigr et al.
Journal of pharmaceutical and biomedical analysis, 151, 291-300 (2018-02-08)
The presented work describes the development and validation of a rapid UHPLC-UV method using a fused core particle column with an RP-Amide stationary phase for the separation and quantitative analysis of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. Three
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What is the Product No of the guard column for Product No. 565325-U Ascentis RP-Amide HPLC column?
1 answer-
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What solvent should Product No. 565325-U Ascentis RP-Amide HPLC Column be stored in?
1 answer-
Product No. 565325-U should be stored in 100% acetonitrile after any buffers have been flushed out.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can I use Ascentis Express on a UHPLC system?
1 answer-
Yes. Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
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