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Merck

505013

Supelco

Discovery® RP-Amide C16 (5 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

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About This Item

UNSPSC-Code:
41115700
eCl@ss:
32110501

product name

Discovery® RP-AmidC16 HPLC-Säule, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

Agentur

suitable for USP L60

Leistungsmerkmale

endcapped

Hersteller/Markenname

Discovery®

Kennzeichnungsgrad

11% Carbon loading

Parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

Methode(n)

HPLC: suitable

L × ID

15 cm × 4.6 mm

Oberflächenbereich

200 m2/g

Oberflächendeckung

2.6 μmol/m2

Verunreinigungen

<10 ppm metals

Matrix

silica gel, high purity, spherical base material

Aktive Matrixgruppe

amide, alkyl phase

Partikelgröße

5 μm

Porengröße

180 Å

operating pH range

2-8

Anwendung(en)

food and beverages

Trenntechnik

reversed phase

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Allgemeine Beschreibung

Discovery® RP-AmideC16 HPLC Column consists of a stationary phase made up of high-purity C16-silica that includes a polar-embedded amide function attached to the silica surface with a propyl group.

Anwendung

Discovery RP-AmideC16 HPLC Column may be used for the separation of:
  • Impurities in drugs such as closantel, levamisole and azaconazole during their determination by high performance liquid chromatography-diode array detection (HPLC-DAD).
  • Vitamin B6 compounds in food samples during their determination using LC coupled with fluorescence detection (FLD).
  • Vitamin C and other fat soluble vitamins in mature seeds of Lathyrus L. taxa during their identification using HPLC with ultraviolet (UV) detection.
  • Zidovudine in combination with other pharmaceuticals in intravenous fluid mixtures during the determination using HPLC combined with photodiode array (PDA) detector.

Discovery® RP-AmideC16 HPLC column may be used for the separation of:
  • Phenolic compounds, purine alkaloids and theanine in Camellia species by high performance liquid chromatography (HPLC) equipped with photodiode array detector (PDA).
  • Proprietary pharmaceutical compounds using HPLC combined with computer-assisted screening and Plackett−Burman experimental design.
  • Hydrophilic bases by LC with principal component analysis (PCA).
  • 2,4-Dinitrophenylhydrazone derivatives by HPLC equipped with ultraviolet (UV) spectrometric detection.

Leistungsmerkmale und Vorteile

  • Hervorragende Rückhaltung und Auflösung polarer Substanzen
  • Einzigartige Selektivität im Vergleich mit C18
  • Hervorragende Peakform und Effizienz
  • Weniger hydrophob als C18-Säulen
  • Kompatibel mit 100 % wässrigen mobilen Phasen
   

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Rechtliche Hinweise

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Lagerklassenschlüssel

13 - Non Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Die Dokumentenbibliothek aufrufen

HPLC separations of zidovudine and selected pharmaceuticals using a hexadecylsilane amide column.
Caufield WV and Stewart JT
Chromatographia, 54(9-10), 561-568 (2001)
Determination of vitamin B6 compounds in foods using liquid chromatography with post-column derivatization fluorescence detection.
Vinas P, et al.
Chromatographia, 59(5-6), 381-386 (2004)
An improved HPLC method for simultaneous determination of phenolic compounds, purine alkaloids and theanine in Camellia species
Peng L, et al.
J. Food Compos. Anal., 21(7), 559-563 (2008)
Strategy for developing and optimizing liquid chromatography methods in pharmaceutical development using computer-assisted screening and Plackett-Burman experimental design
Li W and Rasmussen HT
Journal of Chromatography A, 1016(2), 165-180 (2003)
Isomerization of aldehyde-2, 4-dinitrophenylhydrazone derivatives and validation of high-performance liquid chromatographic analysis
Uchiyama S, et al.
Journal of Chromatography A, 996(1-2), 95-102 (2003)

Protokolle

Separations of Tetracycline Antibiotics by Reversed Phase HPLC, Using Discovery Columns

Chromatograms

application for HPLCapplication for SPE, application for HPLCapplication for HPLC

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