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Merck

V5764

Sigma-Aldrich

Anti-Vesicular GABA Transporter antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(e):

Anti-VGAT

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.77

Biologische Quelle

rabbit

Konjugat

unconjugated

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Wirksamkeit

2-4 mg per mL effective concentration (determined by immunoblotting, using mouse brain and rat brain extracts)

Mol-Gew.

antigen ~55 kDa

Speziesreaktivität

rat, mouse

Verpackung

pkg of 200 μL

Lagerbedingungen

avoid repeated freeze/thaw cycles (Storage in "frost-free" freezers is not recommended)

Konzentration

~1.0 mg/mL

Methode(n)

microarray: suitable
western blot: 2-4 μg/mL using mouse brain and rat brain extracts (S1 fraction)

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

Allgemeine Beschreibung

Vesicular GABA transporter (VGAT), also known as solute carrier family 32 member 1 (SLC32A1) or vesicular inhibitory amino acid transporter (VIAAT), is encoded by the gene localized on human chromosome 20q11.23. The encoded protein is highly expressed in both γ-aminobutyric acid -ergic (GABAergic) and glycinergic nerve endings.

Immunogen

synthetic peptide corresponding to amino acids 1-20 located at the N-terminus of mouse VGAT. This sequence is identical in rat VGAT and highly conserved (single a.a. substitution) in human VGAT.

Anwendung

Anti-Vesicular GABA Transporter antibody produced in rabbit has been used in:
  • immunohistochemistry
  • western blot
  • immunocytochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Biochem./physiol. Wirkung

Vesicular GABA transporter (VGAT) plays a vital role in loading of presynaptic vesicles with glycine and γ-aminobutyric acid (GABA). VGAT is precisely involved in packing GABA into synaptic vesicles. In addition, it also has a crucial role in inhibitory synaptic transmission. Genetic variations in the gene leads to hyperekplexia.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Forebrain Ischemia Triggers GABAergic System Degeneration in Substantia Nigra at Chronic Stages in Rats
Lin B
Cardiovascular Psychiatry and Neurology (2010)
Distribution and Posttranslational Modification of Synaptic ERα in the Adult Female Rat Hippocampus
Tabatadze N
Endocrinology, 154, 2013-2013 (2013)
A critical role for glycine transporters in hyperexcitability disorders
Harvey RJ
Frontiers in Molecular Neuroscience (2008)
Dopaminergic neurons inhibit striatal output via non-canonical release of GABA
Tritsch NX
Nature, 490, 262-266 (2012)
Cyclin-Dependent Kinase 5 Is Involved in the Phosphorylation of Gephyrin and Clustering of GABAA Receptors at Inhibitory Synapses of Hippocampal Neurons
Kalbouneh H
PLoS ONE, 9 (2014)

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