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MAK442

Sorbitol Assay Kit

Sufficient for 100 colorimetric tests

Synonym(e):

D-Sorbitol Assay Kit

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Größe/SKUVerfügbarkeitPreis
1 kit
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€ 511,00

Über diesen Artikel

NACRES:
NA.84
UNSPSC Code:
12161503

€ 511,00


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Quality Segment

input

biological sample(s)
food(s)
plant sample(s)

application(s)

agriculture
cosmetics
environmental
food and beverages

detection method

colorimetric

relevant disease(s)

diabetes

storage temp.

−20°C

General description

Sorbitol, also known as glucitol, is a sugar alcohol metabolized slowly within the human body. It is derived from glucose through the reduction of an aldehyde group to a hydroxyl group. Excessive accumulation of sorbitol in erythrocytes, retinal cells, and Schwann cells has been linked to various health issues including retinopathy, cataracts, peripheral neuropathy, and diabetes. Sorbitol is primarily sourced from corn syrup and naturally occurs in fruits such as apples, pears, peaches, and prunes. Widely utilized as a sugar substitute and laxative, sorbitol also finds applications in specialty culture media, as well as in healthcare, food, and cosmetic products. Monitoring sorbitol levels in biological samples aids in understanding metabolic pathways and tracking the progression of diabetes.

Application

The Sorbitol Assay Kit may be used for:
  • Metabolism Research
  • Food and Beverage Analysis
  • Diabetes Research

The kit is suitable for the quantitative determination of D-sorbitol and evaluation of drug effects on sorbitol metabolism in biological (e.g. blood), food, beverage and agriculture samples.

Biochem/physiol Actions

The sorbitol assay involves an end-point enzyme coupled MTT/NAD reaction that forms a colored product with an absorption maximum at A565 nm. The increase in absorbance at A565 nm is directly proportional to the sorbitol concentration.

Features and Benefits

  • Fast and Precise Measurement: Achieve results in less than 1 hour, providing rapid analysis with a detection range of 5 - 1000 μM D-sorbitol ensures accurate measurements across a wide range of concentrations.
  • High-Throughput Compatibility: Effortlessly integrate our kit into high-throughput handling systems, optimizing efficiency and ensuring accurate results.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

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Dieser Artikel
MAK434
description

Sufficient for 100 colorimetric tests

description

Sufficient for 100 colorimetric tests

Quality Level

100

Quality Level

100

detection method

colorimetric

detection method

colorimetric

storage temp.

−20°C

storage temp.

−20°C

input

biological sample(s)
food(s)
plant sample(s)

input

cell culture medium
tissue
serum
urine
food(s)
beverage(s)
plasma

application(s)

agriculture
cosmetics
environmental
food and beverages

application(s)

agriculture
cosmetics
environmental
food and beverages

relevant disease(s)

diabetes

relevant disease(s)

gastrointestinal diseases


Lagerklasse

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

  1. What is the specificity of this kit and are there any known interfering compounds? Can this kit reliably distinguish sorbitol from its isomers (mannitol, galactitol)?

    1 answer
    1. This kit has a linear detection range (20 µL sample): 5 to 1000 µM D-sorbitol. This kit has not been tested with sorbitol in a matrix of other sugar alcohols. It is possible that other sugar alcohols, such as mannitol, could contribute to interference.

      Helpful?

Reviews

Active Filters

  1. USA
    • Review 1
    • Votes 0
    3 out of 5 stars.

    Performed okay with modification

    This assay kit only worked well when modifications were made to the listed protocols. I had to dilute the included sorbitol standards by quite a bit more than the technical bulletin specified to get a linear calibration curve. I'd also highly recommend running the assay in triplicate instead of duplicate like the directions specify. When everything was properly diluted I was able to get pretty consistent results in fruit juice.

    Helpful?

    1. Response from Merck KGaA:

      Thank you for your review. We are sorry to hear that your experience did not match your product expectations. We encourage customers who experience problems with our products to contact us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.