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Merck

MAK369

Sigma-Aldrich

Ferric Reducing Antioxidant Power (FRAP) Assay Kit (Colorimetric)

sufficient for 200 colorimetric tests

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About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

Verwendung

sufficient for 200 colorimetric tests

Anwendung(en)

cosmetics
food and beverages

Nachweisverfahren

colorimetric

Relevante Krankheit(en)

neurological disorders; cardiovascular diseases; cancer

Allgemeine Beschreibung

Antioxidants are molecules which act as reducing agents by donating electrons to free radicals to stabilize them and minimize the damage caused by free radicals to DNA, cells and organ systems. Antioxidants include substances such as polyphenols; flavonoids; vitamins and enzymes like glutathione peroxidase and superoxide dismutase. They are known to have beneficial health effects such as lowering the risk of cancer, heart disease and neurodegenerative disorders and are abundantly found in plants, fruits, vegetables, beverages and natural products.

Anwendung


  • Biochemical Characterization of Different Chemical Components of Parthenium hysterophorus and Their Therapeutic Potential against HIV-1 RT and Microbial Growth.: This research article examines the chemical components of Parthenium hysterophorus, utilizing the Ferric Reducing Antioxidant Power (FRAP) Assay Kit (Colorimetric) to assess their antioxidant capabilities. The study highlights the therapeutic potential of these components against HIV-1 reverse transcriptase (RT) and microbial growth, offering new insights into the medicinal properties of the plant (Jaiswal et al., 2022).


Leistungsmerkmale und Vorteile

Compatible with high-throughput handling systems.

Eignung

Suitable for the measurement of antioxidant capacity in fruits, vegetables, beverages (like tea and wine), food products, plant extracts, herbal products, serum and plasma.

Prinzip

Ferric reducing antioxidant power (FRAP) assay is a widely used method that uses antioxidants as reductants in a redox-linked colorimetric reaction, wherein Fe3+ is reduced to Fe2+. Ferric (Fe3+) to ferrous (Fe2+) ion reduction at low pH causes formation of a colored ferrous-probe complex from a colorless ferric-probe complex. The assay is high-throughput adaptable and can detect antioxidant capacities as low as 0.2 mM Fe2+ equivalents.

Ersetzt durch

Produkt-Nr.
Beschreibung
Preisangaben

Piktogramme

Corrosion

Signalwort

Warning

H-Sätze

P-Sätze

Gefahreneinstufungen

Met. Corr. 1

Lagerklassenschlüssel

8A - Combustible corrosive hazardous materials

Flammpunkt (°F)

188.6 °F

Flammpunkt (°C)

87 °C


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Questions

  1. Does the kit come with instructions for using microcuvette volumes instead of microplate volumes?

    1 answer
    1. If the total volume of the reaction in a 96-well plate (200 µL) is suitable for the cuvette being used, the same reaction can be used in the cuvette. However, if the volume is not sufficient, adjustments will need to be made to accommodate the volume change.

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