The standard included in this kit is a 50 mM solution of Trolox ((±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid) in alcohol. The solution may be aliquoted and stored at -20°C to avoid repeated freeze/thaw cycles. However, following dilution in water to the working concentration, the standard should used immediately and not stored for later use. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/114/194/mak334bul.pdf
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 kit | Warenkorb auf Verfügbarkeit prüfen | € 343,00 |
Über diesen Artikel
usage
sufficient for 100 colorimetric tests
input
biological sample(s)
plasma
food(s)
serum
urine
beverage(s)
saliva
application(s)
cosmetics
food and beverages
pharmaceutical
detection method
colorimetric
relevant disease(s)
Alzheimer′s disease; Parkinson′s disease; diabetes; neurological disorders
General description
Application
- Cancer Research
- Diabetes Research
- Drug Discovery
- Neurodegenerative Disease Research
- Oxidative Stress Research
Biochem/physiol Actions
Features and Benefits
Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a 10 minute room temperature reaction, reducing complexity and saving valuable time and effort.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Other Notes
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| detection method colorimetric | detection method colorimetric | detection method colorimetric, fluorometric | detection method colorimetric |
| usage sufficient for 100 colorimetric tests | usage sufficient for 200 tests | usage sufficient for 100 colorimetric or fluorometric tests | usage sufficient for 250 colorimetric tests |
| input biological sample(s) | input - | input beverage(s) | input plasma |
| application(s) cosmetics | application(s) - | application(s) - | application(s) - |
| relevant disease(s) Alzheimer′s disease; Parkinson′s disease; diabetes; neurological disorders | relevant disease(s) - | relevant disease(s) cardiovascular diseases; immunological diseases | relevant disease(s) hematological disorder |
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Lagerklasse
10 - Combustible liquids
flash_point_f
188.6 °F
flash_point_c
87 °C
wgk
WGK 2
Hier finden Sie alle aktuellen Versionen:
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
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¿Es posible preparar varios tubos 1mM del standard a partir de la solución standard proporcionada (50 mM) y almacenarlos a -20ºC o debe prepararse en cada ensayo la solución de 1mM de patrón? La idea es evitar tantos ciclos de congelación/descongelación.
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Me interesa saber el contenido de los reactivos A y B del KIT MAK334
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The compositions of Reagent A and Reagent B are considered proprietary information. However, information related to safety involving these reagents can be found in the product Safety Data Sheet.
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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what all the enzymes it can measure like SOD, CAT, GPX, LPO and GSH activity
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This kit is not capable of determining enzymatic activity. The product is intended for use in measuring total antioxidant capacity (TAC) of a given sample as compared to a standard curve based on serial dilutions of Trolox. The kit principal is based on the reduction of Cu2+ to Cu+. Please see the link below to review additional information in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/114/194/mak334bul.pdfNote that there are other kit products available for measuring the activity of specific enzymes.
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Can this kit be used with tissue extracts? If yes, how to extract the tissue (liver)?
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Yes, this kit is suitable for use with tissue extracts. The tissue preparation recommendations are as follows:
Homogenize tissue samples on ice in cold 1xPBS.
(use approximately 0.5 mL PB per 100 mg of tissue)
Centrifuge at 12,000g for 15 minutes at 4°C.
Remove the supernatant and keep it on ice.
Note that the supernatant may be stored in working aliquots at –80°C until ready for testing.Helpful?
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Is that kit provide instruction for the testing
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The product can be found in the 'DOCUMENTATION' section under 'More Documents'. Please use the links below to access the protocol below:
https://www.sigmaaldrich.com/product/sigma/mak334#product-documentation
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/114/194/mak334bul.pdfHelpful?
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