MAK339

Lipopolysaccharide (LPS) Isolation Kit

sufficient for 10 isolation(s)

• Synonym(e): LPS Extraction Kit
• UNSPSC Code: 12161503

Eigenschaften

• usage: sufficient for 10 isolation(s)
• application(s): sample preservation
• storage temp.: −20°C

Beschreibung

General description

The outer membrane of gram-negative bacteria contains lipopolysaccharide (LPS), a low molecular weight carbohydrate with a molecular mass of 10-20 kDa. It is heterogeneous and composed of O antigen (a repeating glycan polymer), core oligosaccharide (which links the O antigen to Lipid A - the third component, and non-carbohydrate components such as phosphate and amino acids groups. Lipid A, has multiple fatty acids which serve to anchor LPS into the bacterial membrane allowing the O antigen and core oligosaccharide to protrude and contributes to a major part of the toxicity of gram-negative bacteria. Also known as endotoxin, when consumed by animals, LPS induces a strong inflammatory response and/or sepsis. 

Application

Suitable for the isolation of Lipopolysaccharides from outer membrane of gram negative bacteria.

Biochem/physiol Actions

The Lipopolysaccharide (LPS) Isolation Kit uses bacterial membrane lysis buffer and protein digestion to yield micrograms of LPS from bacterial culture (approximately 1-4% of dry weight). This kit does not use chloroform or phenol like traditional methods, and it will yield pure LPS in less than 2 hours that can be easily characterized and quantified.

Sicherheitshinweise

• Piktogramme: GHS08,GHS05
• Signalwort: Danger
• Gefahrencodes: H290,H334
• Sicherheitshinweise: P234 - P261 - P284 - P390 - P501
• Hazard Classifications: Met. Corr. 1 - Resp. Sens. 1
• Lagerklasse: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable