G5663

Glutathione S-Transferase from E. coli

recombinant, expressed in E. coli, buffered aqueous solution

• Synonym(e): Glutathione S-alkenetransferase, Glutathione S-alkyltransferase, Glutathione S-aralkyltransferase, Glutathione S-aryltransferase, Glutathione S-epoxidetransferase, RX: Glutathione R-transferase
• UNSPSC Code: 12352204
• NACRES: NA.53
• MDL number: MFCD01634957
• Recombinant: expressed in E. coli
• Concentration: >0.1 mg/mL, >1 unit/mL

Eigenschaften

• recombinant: expressed in E. coli
• grade: Molecular Biology
• form: buffered aqueous solution
• mol wt: 26 kDa
• concentration: >0.1 mg/mL, >1 unit/mL
• UniProt accession no.: P09488
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: human ... GSTM1(2944)

Beschreibung

General description

Glutathione S-transferase (GST) catalyzes the addition of the glutathione thiol group to a suitable electrophilic species. Enzymatic activities are based on the conjugation of reduced glutathione in the presence of a second substrate.

Application

Suitable for use in ELISA and Western blot applications.

Glutathione S-Transferase (GST) from E. coli has been used in the standard curve preparation for the quantification of GST-V5H6 produced by enzyme-linked immunosorbent assay (ELISA).[1]

Biochem/physiol Actions

Glutathione S-transferase (GST) catalyzes the addition of the glutathione thiol group to a suitable electrophilic species. Enzymatic activities are based on the conjugation of reduced glutathione in the presence of a
second substrate.

In ELISA, 0.5 μg of recombinant glutathione S-transferase is the minimum detectable level of enzyme with an anti-glutatione S-transferase, alkaline phosphatase conjugate.

In immunoblot, 50 ng of recombinant GST is the minimum detectable level of enzyme with an anti-glutathione S-transferase, alkaline phosphatase conjugate.

Glutathione S-transferases (GST) catalyzes the conjugation of reduced glutathione with several substrates, which leads to detoxification. It also serves as transport proteins.

Physical form

GST is supplied as a solution in phosphate buffered saline containing 0.02% sodium azide. Every lot of material supplied will have 5 mg total enzyme, at a concentration greater than 0.1 mg/ml, typically 1.0 mg/ml.

Other Notes

One unit will conjugate 1.0 micromole of 1-chloro-2,4-dinitrobenzene with reduced glutathione per minute at pH 6.5 at 25 deg C.

Disclaimer

Due to the sodium azide content, consult the SDS for information regarding hazards and safe handling practices.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable