F4274

Fluorescein-Isothiocyanat Isomer I

≥97.5% purity (HPLC), powder

• Synonym(e): FITC, Fluorescein-5-Isothiocyanat
• Empirische Formel (Hill-System): C₂₁H₁₁NO₅S
• CAS-Nummer: 3326-32-7
• Molekulargewicht: 389.38
• Beilstein: 1407295
• EG-Nummer: 222-042-0
• MDL-Nummer: MFCD00005063
• UNSPSC-Code: 12171500
• PubChem Substanz-ID: 24894860
• NACRES: NA.47

Eigenschaften

• Produktbezeichnung: Fluorescein-Isothiocyanat Isomer I, ≥97.5% (HPLC)
• Qualitätsniveau: 100
• Assay: ≥97.5% (HPLC)
• Form: powder
• Methode(n): titration: suitable
• Farbe: yellow to very dark yellow
• mp (Schmelzpunkt): >360 °C (lit.)
• Löslichkeit: acetone: soluble 10 mg/mL
• Fluoreszenz: λ_ex 492 nm; λ_em 518 nm (green)
• Anwendung(en): diagnostic assay manufacturing; hematology; histology
• Lagertemp.: 2-8°C
• SMILES String: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• InChIKey: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.[1][2]

Anwendung

Fluorescein isothiocyanate isomer I has been used for the staining of conidia.[3][4]

Reagenz für die FITC-Markierung von Proteinen; Mikrosequenzierung von Proteinen und Peptiden (HPLC)

Verpackung

Bottomless glass bottle. Contents are inside inserted fused cone.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H317,H334
• P-Sätze: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Gefahreneinstufungen: Resp. Sens. 1 - Skin Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Faceshields, Gloves