F4274

Fluorescein-Isothiocyanat Isomer I

≥97.5% purity (HPLC), powder

• Synonym(e): FITC, Fluorescein-5-Isothiocyanat
• Empirische Formel (Hill-System): C₂₁H₁₁NO₅S
• CAS-Nummer: 3326-32-7
• Molekulargewicht: 389.38
• NACRES: NA.47
• PubChem Substance ID: 24894860
• UNSPSC Code: 12171500
• EC Number: 222-042-0
• MDL number: MFCD00005063
• Beilstein/REAXYS Number: 1407295

Eigenschaften

• Produktname: Fluorescein-Isothiocyanat Isomer I, ≥97.5% (HPLC)
• InChI key: MHMNJMPURVTYEJ-UHFFFAOYSA-N
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• SMILES string: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
• assay: ≥97.5% (HPLC)
• form: powder
• technique(s): titration: suitable
• color: yellow to very dark yellow
• mp: >360 °C (lit.)
• solubility: acetone: soluble 10 mg/mL
• fluorescence: λ_ex 492 nm; λ_em 518 nm (green)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• Quality Level: 100

Beschreibung

General description

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.[1][2]

Application

Reagenz für die FITC-Markierung von Proteinen; Mikrosequenzierung von Proteinen und Peptiden (HPLC)

Fluorescein isothiocyanate isomer I has been used for the staining of conidia.[3][4]

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H317,H334
• pcodes: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves