This product has not been tested for labeling bacteria internally, including Staphylococcus aureus. Therefore, no data is available for this application. A literature search is recommended for specific applications.
46950
Fluoreszein-5(6)-isothiocyanat
BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
Fluorescein 5(6)-isothiocyanate
Synonym(e):
FITC
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50 MG
€ 39,60
250 MG
€ 114,00
1 G
€ 346,00
Über diesen Artikel
Empirische Formel (Hill-System):
C21H11NO5S
CAS-Nummer:
Molekulargewicht:
389.38
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
EC Number:
248-207-7
Beilstein/REAXYS Number:
1407295
MDL number:
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Unterstützung erhaltenInChI key
MHMNJMPURVTYEJ-UHFFFAOYSA-N
InChI
1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
SMILES string
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
product line
BioReagent
assay
≥90% (HPLC)
Quality Level
form
powder
mol wt
389.38 g/mol
color
orange to dark orange, yellow to dark yellow
solubility
acetone: 0.1 g/10 mL, clear to very slightly hazy
fluorescence
λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0
suitability
suitable for fluorescence
storage temp.
−20°C
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Verwandte Kategorien
1 of 4
Dieser Artikel | |||
|---|---|---|---|
| suitability suitable for fluorescence | suitability - | suitability - | suitability - |
| fluorescence λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0 | fluorescence - | fluorescence λex 492 nm; λem 518 nm (green) | fluorescence λex 492 nm; λem 518 nm (green) |
| assay ≥90% (HPLC) | assay ≥90% (HPLC) | assay ≥90% (HPLC) | assay ≥97.5% (HPLC) |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| product line BioReagent | product line - | product line - | product line - |
General description
Fluorescein 5(6)-isothiocyanate, also known as FITC, is a weakly acidic hydroxyxanthene whose overall size is small, with both carboxylic acid and isothiocyanate substituents on the pendant phenyl ring. The dye has a moderately sized, nonplanar aromatic system. The isothiocyanate group reacts with nucleophilic substituents (e.g. amino, hydroxyl, thiol) of biomolecules to be attached as a fluorescent label. The maximum emission of the dye is at 519nm in the same solvent.
Application
Für die Fluoreszenzmarkierung von Proteinen (z.B. Immunofluoreszenz); H Protein-Labeling für die Gelchromatographie.
Fluorescein 5(6)-isothiocyanate (FITC) labelled molecules are used in non-microscopic immunoassays and physiological tracing experiments. FITC is widely used in flow cytometry experiments for staining proteins, nuclei, and subnuclear particles. White blood cells (WBCs) are marked with FITC to study their response toward a chemoattractant, eventually being measured.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Lagerklasse
11 - Combustible Solids
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Skin Irrit. 2 - Skin Sens. 1B
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Eddie A James et al.
Arthritis & rheumatology (Hoboken, N.J.), 66(7), 1712-1722 (2014-03-26)
Rheumatoid arthritis (RA) is thought to be a T cell-mediated disease, based on its strong association with HLA class II alleles, clinical responsiveness to T cell-directed therapies, and the presence of CD4+ T cells in rheumatoid joints. The presence of
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Advances in the field of stem cells have led to novel avenues for generating induced pluripotent stem cells (iPSCs) from differentiated somatic cells. iPSCs are typically obtained by the introduction of four factors--OCT4, SOX2, KLF4, and cMYC--via integrating vectors. Here
Yuh-Seog Jung et al.
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The purpose of this study was to characterize the expression of CD200, a membrane protein that functions in immune evasion, to examine its correlations with cancer stem cell (CSC)-like features and analyze its response to chemotherapy and radiation in human
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excuse me， can the product of Fluorescein 5(6)-isothiocyanate be used to lable bacteria，such as Staphylococcus aureus？ thanks
1 answer-
Helpful?
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Is it suitable for fluorescence microscopy?
1 answer-
This product is tested for fluorescent labeling of proteins. While not tested specifically for use in fluorescent microscopy, this compound is routinely used in cell-based studies applying this technique.
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