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Merck

A1227

Sigma-Aldrich

ABTS Enhancer

liquid, for peroxidase detection

Synonym(e):

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Enhancer

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About This Item

UNSPSC-Code:
12352204
NACRES:
NA.83
Preise und Verfügbarkeit sind derzeit nicht verfügbar.

Produktbezeichnung

ABTS Enhancer, for peroxidase detection

Qualitätsniveau

Form

liquid

Lagertemp.

2-8°C

Anwendung

2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) has been used for the determination of laccase activity in Trametes versicolor.[1] It may be suitable for use in enzyme-linked immunosorbent assay (ELISA) for detecting signal strength emitted by cells.[2]

Biochem./physiol. Wirkung

2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) is a non-phenolic substrate.[3] It might exhibit antioxidant property.[4] The detection of the horseradish peroxidase substrate ABTS can be improved by the addition of a chemical enhancer. The ABTS enhancer increases the absorbance of the oxidized product 50-150%. This increase is used to improve assay sensitivity or conserve assay components.

Physikalische Form

Supplied as an 11X concentrate

Piktogramme

Health hazard

Signalwort

Danger

H-Sätze

Gefahreneinstufungen

Aquatic Chronic 3 - Carc. 1B

Lagerklassenschlüssel

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Zulassungslistungen

Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.

EU REACH Annex XVII (Restriction List)

CAS No.

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number

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Die Dokumentenbibliothek aufrufen

Investigations on antioxidant potential of phenolic acids and flavonoids: the common phytochemical ingredients in plants
Singh DP, et al.
Journal of Plant Biochemistry & Physiology (2018)
Mark E Levenstein et al.
Stem cells (Dayton, Ohio), 26(12), 3099-3107 (2008-09-20)
Human embryonic stem (ES) cells can be maintained in an undifferentiated state if the culture medium is first conditioned on a layer of mouse embryonic fibroblast (MEF) feeder cells. Here we show that human ES cell proliferation is coordinated by
Khaled K Abu-Amero et al.
Molecular vision, 17, 2769-2775 (2011-11-09)
To evaluate total antioxidant status (TAS) in the plasma of pseudoexfoliation glaucoma (PEG) patients and to compare this level with a matching control group. Additionally, we aim to investigate the effect of the combined action of the lysyl oxidase-like 1
Manickam Naresh Kumar et al.
Bioresource technology, 244(Pt 1), 353-361 (2017-08-07)
Inhibitor mediated intensified bio-pretreatment (IMBP) technology using natural cellulase inhibitor (NCI) for maximum cellulose recovery from paddy straw was studied. Pretreatment was carried out under solid state condition. Supplementation of 8% NCI in pretreatment medium improves cellulose recovery and delignification
Meng-Hsuan Wu et al.
Scientific reports, 9(1), 9754-9754 (2019-07-07)
Laccases that are tolerant to organic solvents are powerful bio-catalysts with broad applications in biotechnology. Most of these uses must be accomplished at high concentration of organic solvents, during which proteins undergo unfolding, thereby losing enzyme activity. Here we show

Artikel

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Questions

1–3 of 3 Questions  
  1. Why should Product A1227, ABTS Enhancer, be used?

    1 answer
    1. This enhancer may be used to increase sensitivity or to conserve assay components.

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. How can Product A1227, ABTS Enhancer, be used?

    1 answer
    1. This product is for microwell applications such as ELISA. A soluble reaction product is produced, so this is not for applications that require a precipitation reaction such as blotting or histochemical applications.

      Helpful?

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