Trizma wird bei der Formulierung von Pufferlösungen im pH-Bereich von 7,5 bis 8,5 verwendet. Tris-Pufferlösungen werden überwiegend in der Zell- und Molekularbiologie bei Verfahren wie der Extraktion und Aufreinigung von Proteinen und Nukleinsäuren eingesetzt. Tris-gepufferte Kochsalzlösung mit Tween 20, pH-Wert 7,6, wird als Waschpuffer in Assays wie Enzymimmunoassays eingesetzt.
Rekonstituierung
Eine (1) in 500 mL entionisiertem Wasser gelöste Tabelle ergibt: 0,15 M Natriumchlorid, 0,050 M TRIS-HCl-Puffer; 0,05 % Tween® 20; pH-Wert 7,6 bei 25 °C
Rechtliche Hinweise
TWEEN is a registered trademark of Croda International PLC
Autoantibodies against prothrombin, including lupus anticoagulant antibodies (LAC), have been identified in patients with systemic lupus erythematosus (SLE) and antiphospholipid syndrome (APS). To identify the epitopes of LAC in patients with SLE and APS, we analyzed B cell epitopes of
Journal of immunoassay & immunochemistry, 26(3), 179-188 (2005-07-14)
Immunogenic glycolipids from the cell wall of Mycobacterium tuberculosis are potential capture antigens in enzyme-linked immunosorbent assays (ELISAs) for the serodiagnostis of tuberculosis. Typically, washing steps in ELISAs are performed with buffers containing a detergent. However, Tween-20, the most commonly
Typical cerebral autosomal-dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is caused by mutations in the human NOTCH3 gene. Cerebral autosomal-dominant arteriopathy with subcortical infarcts and leukoencephalopathy is characterized by subcortical ischemic strokes due to severe arteriopathy and fibrotic thickening
Cutaneous inflammation from UV radiation exposure causes epidermal damage, cellular infiltration, and secretion of pro-inflammatory mediators that exacerbate tissue destruction. Recovery is mediated chiefly by anti-inflammatory M2 macrophages that suppress inflammation and augment epidermal regeneration. Vitamin D enables anti-inflammation to
The microbial adaptive immune system CRISPR mediates defense against foreign genetic elements through two classes of RNA-guided nuclease effectors. Class 1 effectors utilize multi-protein complexes, whereas class 2 effectors rely on single-component effector proteins such as the well-characterized Cas9. Here
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..