This product is a mixture of human serum albumin (HSA) and human gamma-globulin, the latter of which is essentially all human IgG. The E1% (at 280 nm) values for HSA and human IgG are 14 and 5.3, respectively. The lot-specific extinction coefficient for the mixture can be calculated using the protein concentrations provided the certificate of analysis (CofA)._x000D_
Sample calculation, for Lot SLCN8399:_x000D_
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P8119-BULKSLCN8399.pdf_x000D_
The CofA reports an HSA concentration of 4.9 g/dL, and (by difference) a gamma-globulin concentration of 3.3 g/dL. The extinction coefficient at 280 nm for this 8.2 g/dL (82 mg/mL) lot is 86. Here is the calculation: (4.9 X 14) + (3.3 X 5.3) = 86.09, which would round to 86. If the standard were diluted by a factor of 8.2 to give a 1 g/dL or 1% solution, the E1% would be 10.5.
P8119
Protein-Standard
analytical standard, 80 mg/mL (HSA and gamma-globulins)
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5 ML
€ 123,00
About This Item
UNSPSC-Code:
85151701
NACRES:
NA.24
Empfohlene Produkte
Biologische Quelle
human
Qualitätsniveau
Qualität
analytical standard
Form
liquid
Enthält
0.05% sodium azide as preservative
chemische Klasse(n) des Analyten
amino acids, peptides, proteins
Konzentration
80 mg/mL (HSA and gamma-globulins)
Methode(n)
gel permeation chromatography (GPC): suitable
Anwendung(en)
food and beverages
Format
multi-component solution
Lagertemp.
2-8°C
Verwandte Kategorien
Anwendung
Protein standard has been used in the determination of protein content of cod trypsin isoenzymes using bio-rad protein assay and ultraviolet-visible light spectrophotometry (UV-Vis).[1]
Sonstige Hinweise
Humanserum-Albumin und gamma-Globuline
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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What is the extinction coefficient for this 80 mg/mL protein standard?
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