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P1754

Sigma-Aldrich

TWEEN® 80

TWEEN® 80

viscous liquid

Synonym(e):

TWEEN® 80, POE (20) Sorbitanmonooleat, Polyethylenglycolsorbitanmonooleat, Polyoxyethylen-Sorbitan-Monooleat, Polysorbat 80

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise

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25 ML
€ 36,00
500 ML
€ 73,30
1 L
€ 125,00
1 GAL
€ 375,00

€ 36,00

Voraussichtliches Versanddatum10. April 2025

Bulk-Bestellung anfordern
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25 ML
€ 36,00
500 ML
€ 73,30
1 L
€ 125,00
1 GAL
€ 375,00

About This Item

CAS-Nummer:
9005-65-6
MDL-Nummer:
MFCD00082107
UNSPSC-Code:
12161900
NACRES:
NA.21

€ 36,00

Voraussichtliches Versanddatum10. April 2025

Bulk-Bestellung anfordern

Biologische Quelle

Porcine (tallow)
bovine (tallow)

Qualitätsniveau

200

Beschreibung

non-ionic

Form

viscous liquid

Mol-Gew.

micellar avg mol wt 79,000
average mol wt 1310

Zusammensetzung

Oleic acid, ≥58.0% (balance primarily linoleic, palmitic, and stearic acids)

Aggregatnummer

60

CMC

0.012 mM (20-25°C)

Viskosität

400-620 mPa.s

Übergangstemp.

cloud point 65 °C

Löslichkeit

water: soluble, clear to slightly hazy, colorless to yellow

Dichte

1.064 g/cm3

HLB

15

Anwendung(en)

cell analysis

SMILES String

CCCCCCCC/C=C/CCCCCCCC(=O)OCCOCC(C1C(CC(O1)OCCO)OCCO)OCCO

InChI

1S/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-27-29(39-22-19-34)32-28(38-21-18-33)26-31(42-32)41-23-20-35/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9+

InChIKey

RGPBUVUVZKQNHD-MDZDMXLPSA-N

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Allgemeine Beschreibung

Tween® 80 ist ein nichtionisches Detergens und Tensid, das als Ester von Sorbitan und Ölsäure klassifiziert ist. Es handelt sich um eine vielseitige Verbindung, die häufig in verschiedenen biologischen Anwendungen eingesetzt wird, einschließlich Zelllyse, Isolierung von Zellkernen und Zellfraktionierung. Darüber hinaus wird Tween® 80 zur Stabilisierung von Proteinen und zur Unterstützung bei Proteinmembranstudien eingesetzt. Seine Wirksamkeit ist auf seine Zugehörigkeit zur Polysorbat-Gruppe zurückzuführen, die durch die Kombination von Sorbitol- und Sorbitan-Ölsäureestern mit Polyethylenglykolketten gekennzeichnet ist. Diese strukturellen Merkmale tragen zu den Emulgatoreigenschaften von Tween® 80 und einer Vielzahl von Anwendungen bei.

Anwendung

Nichtionisches Detergens zur selektiven Proteinextraktion und Isolierung von Zellkernen aus Säugerzelllinien.
TWEEN® 80 wird als Trägerstofflösung für spezifische Injektionen in Mäusen und Ratten genutzt.[1][2] Es wird für die Bestimmung der Esterase-Aktivität in Bakterienzellen verwendet.[3]

Biochem./physiol. Wirkung

TWEEN® 80 ist ein Polysorbat-Tensid, das zur Stabilisierung von Proteinen beiträgt und für Proteinmembran-Studien hilfreich ist. Es wird regelmäßig zur Extraktion von Membranproteinen und zur Isolierung von Zellkernen verwendet.[4][5][6]

Leistungsmerkmale und Vorteile

  • Ausgezeichnetes Benetzungs- und Disperionsmittel
  • Vielseitig einsetzbares Tensid für Ihre Forschung in den Bereichen Zellbiologie und Biochemie

Sonstige Hinweise

Füllen Sie bitte dieses Formular aus, um weitere Informationen zu unserem Sortiment an Biochemikalien zu erhalten.

Rechtliche Hinweise

TWEEN is a registered trademark of Croda International PLC

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Beschreibung
Preisangaben

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TWEEN® 80, BioXtra, viscous liquid

P5188

TWEEN® 80, for molecular biology, syrup

P6474

TWEEN® 80, viscous liquid, Preservative Free, Low-peroxide; Low-carbonyls

P6349

TWEEN® 80, viscous liquid, Low-peroxide

P6224

TWEEN® 80, from non-animal source

P4780

TWEEN® 80, suitable for cell culture, suitable for insect cell culture, viscous liquid

P8192

TWEEN® 80 -Lösung, 10%, low peroxide

59924

Polysorbat 80, tested according to Ph. Eur.

STS0204

ECO TWEEN® 80, viscous liquid

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

>235.4 °F - closed cup

Flammpunkt (°C)

> 113 °C - closed cup

Persönliche Schutzausrüstung

Eyeshields, Gloves

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
BCCM7843
BCCM6321
BCCM3263
BCCL4939
BCCL8221

Die passende Version wird nicht angezeigt?

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Suche nach COA

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W291706

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TWEEN® 80 (Polysorbat) EMPROVE® ESSENTIAL, Ph. Eur., JP, NF

SAFC

8.17061

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Sigma-Aldrich

P1504

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Zandra Gerdes et al.
PloS one, 14(2), e0205378-e0205378 (2019-02-20)
The role of microplastic (MP) as a carrier of persistent organic pollutants (POPs) to aquatic organisms has been a topic of debate. However, the reverse POP transport can occur if relative contaminant concentrations are higher in the organism than in
HU-210 shows higher efficacy and potency than morphine after intrathecal administration in the mouse formalin test.
Guhring H et al.
European Journal of Pharmacology, 429, 127-127 (2001)
V Ryu et al.
Neuroscience, 167(4), 1227-1238 (2010-03-04)
Previous reports show that vagal afferent innervation of the stomach eventually regenerates from surviving nodose ganglion (NG) neurons after subdiaphragmatic vagotomy. Systemic capsaicin treatment destroys gastric vagal afferent neurons expressing vanilloid receptor 1 (VR1). However, it is not known whether
Yee-Kuen Yam et al.
Frontiers in microbiology, 11, 359-359 (2020-03-21)
Persistence of infection despite extensive chemotherapy with antibiotics displaying low MICs is a hallmark of lung disease caused by Mycobacterium abscessus (Mab). Thus, the classical MIC assay is a poor predictor of clinical outcome. Discovery of more efficacious antibiotics requires
Davidson JN
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1 Review This action will navigate to reviews.

Questions

1–10 of 11 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. This material is free of residual solvents?

    1 answer

    1. This product is not tested for residual solvents. Should this be a requirement, see product 817061, which is tested for Ethylene Oxide, 1,4-Dioxane, and Ethylene glycol. Please see the link below to review this item:
      https://www.sigmaaldrich.com/product/mm/817061

      Helpful?

  4. What is the boiling point of this specific Tween 80 product?

    1 answer

    1. This product is not tested for boiling point. However, various reference sources cite a boiling point of approximately 100°C.

      Helpful?

  5. Hello, Is there an expiration date or retest date for this product?

    1 answer

    1. This product is not assigned an expiration date. It is assigned a recommended retest date of approximately 4 years after the quality release date. A recommended retest date is the time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications.

      For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  6. Is Product P1754, TWEEN® 80, cell culture tested?

    1 answer

    1. No, P1754 is not cell culture tested, but product P4780 is cell culture tested TWEEN 80.

      Helpful?

  7. What is the Critical Micelle Concentration (CMC) of Product P1754, TWEEN® 80?

    1 answer

    1. The CMC value is 13-15 mg/Liter.

      Helpful?

  8. Is Product P1754, TWEEN® 80, tested for RNase and DNase activity?

    1 answer

    1. No. Product P1754 is not tested for contaminating RNase and DNase activity.  Product P5188 is a Molecular Biology grade product that has been tested for DNase and RNase.

      Helpful?

  9. Is it normal for the color of Product P1754, TWEEN® 80, to change from lot to lot?

    1 answer

    1. Yes. According to Sigma-Aldrich's specifications, Product No. P1754 can appear bright yellow to yellow with a green cast.  Please see our Specification Sheet for more information.

      Helpful?

  10. Is Product P1754, TWEEN® 80, sterile?

    1 answer

    1. The product is not sterile. Autoclaving a solution of TWEEN 80 is generally not advised. Sterile filtration is more easily done if the liquid is warmed to about 40°C and alternate portions of hot distilled water and TWEEN 80 are poured through the 0.22 μm filter. The TWEEN 80 will blend and remain in solution.

      Helpful?

1–10 of 11 Questions  

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