N6639

Nitrotetrazolium-Blauchlorid

powder, electrophoresis grade

• Synonym(e): 2,2′-Bis-(4-nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylen)-ditetrazoliumchlorid, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylen)-bis-[2-(4-nitrophenyl)-5-phenyl-2H-tetrazoliumchlorid], p-Nitroblau-Tetrazoliumchlorid, p-Nitrotetrazoliumblau, NBT, Nitro BT
• Lineare Formel: C₄₀H₃₀N₁₀O₆ · 2Cl
• CAS-Nummer: 298-83-9
• Molekulargewicht: 817.64
• Beilstein: 4115923
• EG-Nummer: 206-067-4
• MDL-Nummer: MFCD00012159
• UNSPSC-Code: 12171500
• PubChem Substanz-ID: 24897786
• NACRES: NA.52

Eigenschaften

• Qualität: electrophoresis grade
• Qualitätsniveau: 100
• Assay: >90%
• Form: powder
• Methode(n): electrophoresis: suitable
• Löslichkeit: H₂O: 10 mg/mL (A stock solution at 10mg/ml in water is stable for 1-2 weeks in the dark at 0-4°C.); 2-methoxyethanol: 20 mg/mL; ethanol: 5 mg/mL
• Lagertemp.: 2-8°C
• SMILES String: [Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8
• InChI: 1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
• InChIKey: FSVCQIDHPKZJSO-UHFFFAOYSA-L

Beschreibung

Allgemeine Beschreibung

Nitrotetrazolium Blue chloride (NBT) is a dye that is used for detection of alkaline phosphatase in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP).

Anwendung

Nitrotetrazolium Blue chloride has been used:

• to determine the superoxide anion in skeletal muscle cells
• as a component in Tris-HCl buffered saline for nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry of brain sections
• as a chromogenic substrate to study the histochemical detection of hydrogen peroxide (H2O2) and superoxide (O₂- ) anion in Brassica juncea seedlings
• as a component in Triton X-100 solution to perform staining of coronal brain sections

Biochem./physiol. Wirkung

Nitrotetrazolium Blue chloride (NBT) has also been used as a colorimetric indicator of bacterial infection in blood samples. NBT has also been used as a redox indicator for other enzymatic reactions. NBT produces a dark blue insoluble formazan compound while reacting with O₂.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H302,H315,H319,H335
• P-Sätze: P261 - P264 - P270 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Gefahreneinstufungen: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Zielorgane: Respiratory system
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)