10197734001

Glutamate Dehydrogenase (GlDH)

from beef liver

• Synonym(e): DNA marker
• EC-Nummer: 1.4.1.3 (BRENDA, IUBMB)
• UNSPSC-Code: 12352204

Eigenschaften

• Biologische Quelle: bovine liver
• Qualitätsniveau: 100
• Form: lyophilized
• Spezifische Aktivität: 10 U/mg; ~10 units/mg protein (at 25 °C with 2-oxoglutarate as the substrate, and ADP as the activator.)
• Verpackung: pkg of 3,000 U
• Hersteller/Markenname: Roche
• Konzentration: ≥10-20 % (w/w)
• Methode(n): activity assay: suitable
• Farbe: white
• Optimaler pH-Wert: 8
• Löslichkeit: water: 20 mg/mL, colorless
• Eignung: suitable for UV spectrophotometry and general use
• UniProt-Hinterlegungsnummer: P00366
• Anwendung(en): life science and biopharma
• Fremdaktivität: ADH <0.00500%; LDH <0.00500%; MDH <0.00500%
• Versandbedingung: wet ice
• Lagertemp.: 2-8°C
• Angaben zum Gen: bovine ... GLUD1(281785)

Beschreibung

Allgemeine Beschreibung

Approximately 10 U/mg lyophilizate (120 U/mg enzyme protein) at +25°C with 2-oxoglutarate as the substrate, and ADP as the activator.

GDH (Glutamate dehydrogenase) is a hexamer of 449 residues and is located in the mitochondria. It acts as a branch-point enzyme between amino acid oxidation and urea production. L-glutamate:NAD(P)⁺ oxidoreductase is involved in deamination.

Anwendung

Glutamate dehydrogenase has been used to measure residual ammonium by enzymatic analysis during fermentation by wine yeast. It used as a component of cofactor recycling systems for NAD(P) and NAD(P)H.

Qualität

Contaminants: <0.005% ADH, LDH, and MDH, each

Sequenz

The smallest active structure of GIDH (310,000 to 350,000 ) is a hexamer subunit of 55,400 D. This hexamer reversibly self-assembles forming a polymer with up to eight hexamers (2,200 kD).

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): does not flash
• Flammpunkt (°C): does not flash