1.04122

Methylenblau

• Synonym(e): Methylene Blue trihydrate
• Empirische Formel (Hill-System): C₁₆H₁₈ClN₃S · 3H₂O
• CAS-Nummer: 7220-79-3
• Molekulargewicht: 373.90
• Beilstein: 3599847
• MDL-Nummer: MFCD00012111
• UNSPSC-Code: 41116130

Eigenschaften

• Form: solid
• Qualitätsniveau: 200
• Wirksamkeit: 1180 mg/kg LD₅₀, oral (Rat)
• Zusammensetzung: Dye, ≥82% spectrophotometric assay
• pH-Wert: 3 (20 °C, 10 g/L in H₂O)
• mp (Schmelzpunkt): 190 °C (dec.) (lit.)
• Löslichkeit: 50 g/L
• Dichte: 1.757 g/cm³ at 22 °C
• Schüttdichte: 400‑600 kg/m³
• Anwendung(en): diagnostic assay manufacturing; hematology; histology
• Lagertemp.: 2-30°C
• SMILES String: [Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1
• InChIKey: CXKWCBBOMKCUKX-UHFFFAOYSA-M

Beschreibung

Allgemeine Beschreibung

Methylene blue (C.I. 52015) for microscopy Certistain^® is a dry dye that is used in bacteriology for an overview stain and as counterstaining for Ziehl-Neelsen method in sample material of human origin. Bacteria appear blue. The dye has a further important application in hematology: Methylene blue and eosin are the main components of many staining solutions and dye mixtures. As a CERTISTAIN^® dye it is chemically quality-certified according to highest standards and strict specifications.

Anwendung

for microscopy Certistain^®

Hinweis zur Analyse

Dye content (spectrophotometrically): ≥ 82 %
Identity
- Chloride:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (ethanol 50 %): 660 - 665 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.003 g/l; ethanol 50 %): 2250 - 2750
TLC-Test: passes test
Loss on drying (110 °C): 10 - 15 %
Suitability for microscopy: passes test

Rechtliche Hinweise

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H302
• P-Sätze: P264 - P270 - P301 + P312 - P501
• Gefahreneinstufungen: Acute Tox. 4 Oral
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 2