03978

Methylenblau -Lösung

for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution

• Synonym(e): Ehrlichs Reagens III, Loeffler’s Methylenblau
• Empirische Formel (Hill-System): C₁₆H₁₈ClN₃S
• CAS-Nummer: 61-73-4
• Molekulargewicht: 319.85
• Beilstein: 3641570
• MDL-Nummer: MFCD00012111
• UNSPSC-Code: 12171500
• PubChem Substanz-ID: 329747853
• NACRES: NA.47

Eigenschaften

• Qualität: for microscopy; concentrated, aqueous solution
• Form: liquid
• Haltbarkeit: ~3 yr
• Konzentration: 1.5% (1.5 g/ 100 mL)
• Dichte: 1.0 g/mL at 20 °C
• εmax: 9.4 at 656-666 nm in water
• Anwendung(en): diagnostic assay manufacturing; hematology; histology
• Lagertemp.: room temp
• SMILES String: [Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1
• InChIKey: CXKWCBBOMKCUKX-UHFFFAOYSA-M

Beschreibung

Allgemeine Beschreibung

Methylene blue is used to stain animal cells to make their nuclei more observable. It is a component of staining media such as Wright′s stain and Jenner′s stain.

Methylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye. It is a small molecular weight dye, which is effective against Gram positive and Gram negative bacteria.

Anwendung

Methylene Blue solution has been used to stain human amniotic fluid stem cells to determine cell viability. It has also been used to stain sections to determine the presence of mycobacteria.

Methylene blue is used to stain blood films/smears used in cytology and to stain RNA or DNA for viewing under the microscope or on hybridization membranes.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter