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Sigma-Aldrich

DST (disuccinimidyl tartrate)

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About This Item

Empirische Formel (Hill-System):
C12H12O10N2
CAS-Nummer:
62069-75-4
Molekulargewicht:
344.23
MDL-Nummer:
MFCD00049060
UNSPSC-Code:
12352200
PubChem Substanz-ID:
329768802

Assay

≥85%

Form

powder

Mol-Gew.

344.24

Eignung der Reaktion

reagent type: cross-linking reagent

Lagerbedingungen

desiccated

Löslichkeit

DMSO or DMF: soluble

Versandbedingung

ambient

Lagertemp.

2-8°C

SMILES String

O=C(C(O)C(O)C(ON1C(CCC1=O)=O)=O)ON2C(CCC2=O)=O

InChI

1S/C12H12N2O10/c15-5-1-2-6(16)13(5)23-11(21)9(19)10(20)12(22)24-14-7(17)3-4-8(14)18/h9-10,19-20H,1-4H2

InChIKey

NXVYSVARUKNFNF-UHFFFAOYSA-N

Allgemeine Beschreibung

DST is a homobifunctional crosslinker that contains amine-reactive N-hydroxysuccinimide (NHS) ester groups and is periodate cleavable. DST is commonly used for conjugating radiolabeled ligands to cell surface receptors. DST must first be dissolved in an organic solvent, such as DMSO or DMF, then added to the aqueous reaction mixture. DST is lipophilic, membrane-permeable and does not possess a charged group, which makes it useful for intracellular and intramembrane protein conjugation.

Leistungsmerkmale und Vorteile

  • Reactive groups: NHS ester (both ends)
  • Reactive towards: amino groups (primary amines)
  • Soluble in DMSO or DMF at a concentration of ~10 mM (3 mg/mL)
  • Cleavable by sodium meta-periodate (Part No. 20504)
  • Ideal for applications in which crosslink cleavability is desired without disturbing protein disulfide bonds

Vorsicht

This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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R J Smith et al.
Biochemistry, 17(18), 3719-3723 (1978-09-05)
Two novel cross-linkers, disuccinimidyl tartarate (DST) and N,N'-bis(3-succinimidyloxycarbonylpropyl)tartaramide (SPT), have been synthesized. These reagents span 6 and 18 A, respectively, between functional groups and contain a vic-glycol bond which can be cleaved with periodate under mild reaction conditions. Both DST
T C Farries et al.
Journal of immunology (Baltimore, Md. : 1950), 142(3), 842-847 (1989-02-01)
Properdin (P) is synthesized by the human promyelocytic cell line, HL-60, after differentiation with DMSO. The secreted P was physiochemically indistinguishable from purified plasma P. It was polymerized and able to bind to C3IBb-Sepharose but not to C3i-Sepharose. No extracellular
L S Park et al.
The Journal of biological chemistry, 261(1), 205-210 (1986-01-05)
125I-Labeled colony-stimulating factor (CSF) 2 alpha (interleukin 3, multi-CSF, and mast cell growth factor) was used to characterize receptors specific for this lymphokine on the cell surface of the factor-dependent cell line FDC-P2. CSF-2 alpha binding to these cells was
J Carlsson et al.
The Biochemical journal, 173(3), 723-737 (1978-09-01)
A heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate, was synthesized. Its N-hydroxysuccinimide ester group reacts with amino groups and the 2-pyridyl disulphide structure reacts with aliphatic thiols. A new thiolation procedure for proteins is based on this reagent. The procedure involves two steps.

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