An anti-pan-protein-specific antibody will recognize the specific protein regardless of phosphorylation state (or other possible post-translational modification). An anti-phospho-protein specific antibody will recognize the protein only in a phosphorylated state.
RAB0352
Cell-Based ELISA Sampler Kit for detecting phospho-ERK1/2 (pThr202/pTyr204), phospho-JNK (pThr183/pTyr185), and phospho-p38 MAPK (pThr180/pTyr182) in cultured cell lines
192 assays
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About This Item
UNSPSC Code:
41116158
NACRES:
NA.32
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usage
192 assays
species reactivity
mouse, human, rat
technique(s)
ELISA: suitable
capture ELISA: suitable
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... MAPK1(5594) , MAPK14(1432) , MAPK3(5595) , MAPK8(5599)
mouse ... MAPK1(26413) , MAPK14(26416) , MAPK3(26417) , MAPK8(26419)
rat ... MAPK1(116590) , MAPK14(81649) , MAPK3(50689) , MAPK8(116554)
General description
The Cell-Based ERK1/2 (activated) ELISA Sampler Kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of ERK1/2 (pThr202 / pTyr204), JNK (pThr183 / pTyr185) and p38 MAPK (pThr180m / pTyr182) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines.
Immunogen
ERK1/2 (pThr202 / pTyr204), JNK (pThr183 / pTyr185), p38 MAPK (pThr180 / pTyr182) synthetic phosphopeptides
Application
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Los componentes del kit también están disponibles por separado
Referencia del producto
Descripción
SDS
- RABBLOCK5x Blocking Solution (Item F)SDS
- RABFIX1Fixing Solution (Item D)SDS
- RABHRP1HRP-conjugated Anti-Rabbit IgG Concentrate (Item I1)SDS
- RABHRP2HRP-conjugated Anti-Mouse IgG Concentrate (Item I2)SDS
- RABPLATE1Uncoated MicroplateSDS
- RABQUENCHQuenching Solution for Cell-based ELISA Assay (Item E)SDS
- RABSTOP1Stop SolutionSDS
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Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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Xiaoqing Zhang et al.
Stem cell research, 32, 115-125 (2018-10-03)
Vascular smooth muscle cells (VSMCs) play essential roles in regulating blood vessel form and function and they are required for vascular tissue regeneration. Multipotent adipose derived stromal cells (ASCs) can be differentiated into VSMC-like cells, which can be used as
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What does PAN means? What is the difference between PAN and PHOSPHO? This is not described in the protocol
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