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Merck

D8062

DMEM/F-12

With ʟ-glutamine, sodium bicarbonate and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

Sinonimo/i:

DME/F-12, miscela 1:1

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Informazioni su questo articolo

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217417

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Nome del prodotto

Terreno Eagle modificato di Dulbecco/miscela nutritiva Ham F-12, With L-glutamine and sodium bicarbonate, without HEPES, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: 3.15 g/L
NaHCO3: yes
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: 0.055 g/L

shipped in

ambient

storage temp.

2-8°C

Quality Level

400

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Questo articolo
D8437D6421D8900
Sigma-Aldrich

Sigma-Aldrich

D8062

Terreno Eagle modificato di Dulbecco/miscela nutritiva Ham F-12

Sigma-Aldrich

Sigma-Aldrich

D8437

Terreno Eagle modificato di Dulbecco/miscela nutritiva Ham F-12

Terreno Eagle modificato di Dulbecco/miscela nutritiva Ham F-12 With 15 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

D6421

Terreno Eagle modificato di Dulbecco/miscela nutritiva Ham F-12

Sigma-Aldrich

Sigma-Aldrich

D8900

Terreno Eagle modificato di Dulbecco/miscela nutritiva Ham F-12

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

liquid

form

liquid

form

liquid

form

powder

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

components

glucose: 3.15 g/L
NaHCO3: yes
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: 0.055 g/L

components

glucose: 3.15 g/L
HEPES: yes
L-glutamine: yes
phenol red: yes
sodium pyruvate: 0.055 g/L

components

sodium pyruvate: 0.055 g/L
L-glutamine: no
phenol red: yes
NaHCO3: yes
HEPES: 15 mM
glucose: 3.15 g/L

components

sodium pyruvate: 0.055 g/L
HEPES: 15 mM
NaHCO3: no
L-glutamine: yes
glucose: 3.15 g/L
phenol red: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:
  • to maintain human lung and colorectal cancer cell lines for the biological evaluation of the secondary metabolites[1]
  • to culture human kidney-2 (HK-2) cells and as a component of the assay medium for lysophosphatidylcholine (LPC) treatment[2]
  • to culture mouse squamous cell carcinoma (SCCVII) tumor cells[3]

General description

Media are supplemented with a defined mixture of nutrients, growth factors, and hormones instead of serum. A 1:1 mixture of Dulbecco′s Modified Eagle′s medium (DME) and Ham′s F-12 nutrient mixture is commonly used for these defined studies. To compensate for the loss of buffering capacity caused by eliminating serum, a final concentration of 15 mM HEPES buffer can be added to the formulations. It allows the investigator to optimize the buffering system.

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Classe di stoccaggio

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
RNBL6949
RNBL5294
RNBL2018
RNBK9603
RNBK6544

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