DMEM/F-12

Name: DMEM/F-12
Brand: SIGMA

With ʟ-glutamine, sodium bicarbonate and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

Sinonimo/i:

DME/F-12, miscela 1:1

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Informazioni su questo articolo

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00217417

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Proprietà

Nome del prodotto

Terreno Eagle modificato di Dulbecco/miscela nutritiva Ham F-12, With L-glutamine and sodium bicarbonate, without HEPES, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: 3.15 g/L
NaHCO₃: yes
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: 0.055 g/L

shipped in

ambient

storage temp.

2-8°C

Descrizione

General description

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Media are supplemented with a defined mixture of nutrients, growth factors, and hormones instead of serum. A 1:1 mixture of Dulbecco′s Modified Eagle′s medium (DME) and Ham′s F-12 nutrient mixture is commonly used for these defined studies. To compensate for the loss of buffering capacity caused by eliminating serum, a final concentration of 15 mM HEPES buffer can be added to the formulations. It allows the investigator to optimize the buffering system.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:

to maintain human lung and colorectal cancer cell lines for the biological evaluation of the secondary metabolites
to culture human kidney-2 (HK-2) cells and as a component of the assay medium for lysophosphatidylcholine (LPC) treatment
to culture mouse squamous cell carcinoma (SCCVII) tumor cells

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Questo articolo	D8437	D6421	D8900
Sigma-Aldrich D8062 Terreno Eagle modificato di Dulbecco/miscela nutritiva Ham F-12	Sigma-Aldrich D8437 Terreno Eagle modificato di Dulbecco/miscela nutritiva Ham F-12	Sigma-Aldrich D6421 Terreno Eagle modificato di Dulbecco/miscela nutritiva Ham F-12	Sigma-Aldrich D8900 Terreno Eagle modificato di Dulbecco/miscela nutritiva Ham F-12
description With L-glutamine and sodium bicarbonate, without HEPES, liquid, sterile-filtered, suitable for cell culture	description With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture	description With 15 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture	description With L-glutamine and 15 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility -
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form liquid	form liquid	form liquid	form powder
components glucose: 3.15 g/L NaHCO₃: yes L-glutamine: yes phenol red: yes HEPES: no sodium pyruvate: 0.055 g/L	components glucose: 3.15 g/L HEPES: yes L-glutamine: yes phenol red: yes sodium pyruvate: 0.055 g/L	components sodium pyruvate: 0.055 g/L L-glutamine: no phenol red: yes NaHCO₃: yes HEPES: 15 mM glucose: 3.15 g/L	components sodium pyruvate: 0.055 g/L HEPES: 15 mM NaHCO₃: no L-glutamine: yes glucose: 3.15 g/L phenol red: yes
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient