Stock solutions retain activity after 5 freeze-thaw cycles when stored at –20°C. Studies at –80°C have not been conducted. For additional solution stability information. see the product datasheet for the research grade product, P8375.
A link to this document is provided below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/160/616/p8375dat-ms.pdf
推薦產品
描述
For research or further manufacturing use only. Not intended for direct use in humans or animals. Non-TSCA use only in US.
品質等級
比活性
≥250 units/mg solid (using pyrogallol)
適合性
suitable for immunoassay
suitable for manufacturing use
應用
diagnostic assay manufacturing
運輸包裝
wet ice
儲存溫度
2-8°C
SMILES 字串
[O+H2]O[O-]
InChI
1S/H2O3/c1-3-2/h1-2H
InChI 密鑰
JSPLKZUTYZBBKA-UHFFFAOYSA-N
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一般說明
辣根过氧化物酶是从辣根（Amoracia rusticana）中分离出来的，属于过氧化物酶的铁原卟啉基团。HRP是含有四个二硫键的单链多肽。它是一种含有18%碳水化合物的糖蛋白。碳水化合物由半乳糖、阿拉伯糖、木糖、岩藻糖、甘露糖、甘露糖胺和半乳糖胺组成，具体取决于特定同工酶。其分子量（约44 kDa）包括多肽链（33,890道尔顿）、氯化血红素加Ca2+ （约700道尔顿）和碳水化合物（约9,400道尔顿）。至少存在7种HRP同工酶。 辣根过氧化物酶同工酶的等电点范围为3.0-9.0。
Horseradish peroxidase (HRP) is isolated from horseradish roots (Amoracia rusticana). HRP is a single chain polypeptide containing four disulfide bonds. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes is 3.0 - 9.0.
應用
来自辣根的过氧化物酶已用于免疫印迹。[1]
HRP is used in immunoassays, such as ELISAs, Western blots, and lateral flow assays. HRP is used in academic research, diagnostics, and pharmaceutical research. For more applications, see the following technical article: An Introduction to Horseradish Peroxidase (HRP) and Its Applications.
單位定義
一个连苯三酚单元将在20℃、pH 6.0条件下，在20秒内从连苯三酚形成1.0mg的红棓酚。
分析報告
RZ(Reinheitszahl)是指溶于去离子水中的酶(0.5–1.0 mg/ml)的吸光度比值A403/A275 。 它是血红素含量的量度，而不是酶活性。 即使是RZ值较高的制剂也可能具有较低的酶活性。
其他說明
有关过氧化物酶的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
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文章
Horseradish peroxidase (HRP) is an enzyme used for detecting molecules, degrading toxins, cross-linking proteins, and in diagnostics like lateral flow assays.
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What is the stability of the activity after reconstituted into PBS if stored at -20 or -80C?
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