推薦產品
生物源
bovine lung
yeast
形狀
lyophilized powder
儲存溫度
−20°C
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特異性
抑制丝氨酸、半胱氨酸和金属蛋白酶。
應用
已针对一般用途进行了优化和测试。
蛋白酶抑制剂混合物粉末已用于免疫印迹和免疫沉淀中。[1]
蛋白酶抑制剂混合物粉末已用于免疫印迹和免疫沉淀中。[1]
生化/生理作用
该混合物含有单独的组分,包括2mM的AEBSF、0.3 μ的抑肽酶、116 μM的乌苯美司、14 μM的E-64、1 μM的亮肽素和1mM的EDTA。每种组分都具有特定的抑制特性。
AEBSF和抑肽酶可以抑制丝氨酸蛋白酶,包括胰蛋白酶、胰凝乳蛋白酶和纤溶酶等。乌苯美司可抑制氨肽酶。E-64可抑制半胱氨酸蛋白酶。EDTA是一种金属蛋白酶的抑制剂。亮肽素作用于丝氨酸和半胱氨酸蛋白酶。
蛋白酶有助于将蛋白质分解成可溶性肽和氨基酸。[2]
AEBSF和抑肽酶可以抑制丝氨酸蛋白酶,包括胰蛋白酶、胰凝乳蛋白酶和纤溶酶等。乌苯美司可抑制氨肽酶。E-64可抑制半胱氨酸蛋白酶。EDTA是一种金属蛋白酶的抑制剂。亮肽素作用于丝氨酸和半胱氨酸蛋白酶。
蛋白酶有助于将蛋白质分解成可溶性肽和氨基酸。[2]
成分
1瓶血清瓶
组分
AEBSF
抑肽酶
乌苯美司
E-64
EDTA
亮抑酶肽
AEBSF
抑肽酶
乌苯美司
E-64
EDTA
亮抑酶肽
數量
一瓶可制备100mL混合液,其足以用于抑制约20克细胞提取物中的蛋白酶。
并非所有提取物都含有相同水平的内源酶,并且可能需要调整所需的混合液用量。
并非所有提取物都含有相同水平的内源酶,并且可能需要调整所需的混合液用量。
準備報告
该产品以冻干粉形式提供。一瓶可制备100毫升混合液。
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2 - Skin Irrit. 2
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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