跳轉至內容
Merck
全部照片(1)

重要文件

QPBCA

Sigma-Aldrich

QuantiPro ™ BCA 检测试剂盒

for 0.5-30 μg/ml protein

同義詞:

BCA Assay, Protein determination by BCA, bicinchoninic acid reagent

登入查看組織和合約定價

選擇尺寸

1 KIT

登入查看價格

履行和交付延遲

全部照片(1)

選擇尺寸

變更視圖
1 KIT

About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

登入查看價格

履行和交付延遲

運輸包裝

wet ice

儲存溫度

2-8°C

相關類別

應用

该试剂盒可在极少量样品中测量极低的蛋白浓度。准确测量蛋白浓度的范围，试管测定为0.5至30 μg/mL；96孔或 384孔平板测定则为1至20 μg/mL。
除了在溶液中测定和定量蛋白质外，[1][2][3] QuantiPro™ BCA分析试剂盒还用于其他应用，包括测定与琼脂糖载体共价结合的蛋白质和吸附在多孔板上的蛋白质。 也用于测定各种固体载体上的官能团，如巯基、 N-羟基琥珀酰亚胺基羧酸盐、醛和酰肼。

特點和優勢

  • 在广泛的蛋白浓度范围内均能维持高精确度
  • 灵敏度高；蛋白浓度介于0.5 至 30 μg/ml之间时，具有线性响应
  • 稳定的彩色复合物
  • 降低了去垢剂的影响

原則

蛋白质在碱性条件下将Cu2+还原为Cu1+ ，形成二辛可宁酸:1+复合物，该复合物在562 nm处有最大吸光度。

儲存和穩定性

室温下储存试剂QA、QB和QC。不使用试剂 QC（4％ 五水硫酸铜（II）溶液12mL），仅将试剂QA和QB混合，置于密闭容器，可在室温下保存一周。如果本试剂盒遇冷或冷藏，则在试剂QA或试剂QB中可能会形成沉淀。要溶解沉淀，请在混合的同时将溶液温度缓慢升至室温。该溶液现在适合使用。

法律資訊

QuantiPro is a trademark of Sigma-Aldrich Co. LLC

僅套裝組件

產品號碼
描述
  • Copper(II) sulfate solution, 4 % (w/v) (prepared from copper (II) sulfate pentahydrate) 12 mL

套裝中的組件也可單獨購買

產品號碼
描述
SDS
  • P0914Protein Standard Solution: 1.0 mg/ml bovine serum albumin in 0.15 M NaCl with 0.05% sodium azide (flame-sealed glass ampules) 10 x 1SDS

應用

產品號碼
描述
訂價

相關產品

產品號碼
描述
訂價

象形圖

CorrosionEnvironment
GHS05,GHS09

訊號詞

Danger

危險分類

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Irrit. 2

儲存類別代碼

8A - Combustible corrosive hazardous materials

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
0000431772
0000431772
0000395428
0000364131
0000340841

未看到正確版本？

如果您需要一個特定的版本，您可以透過批號來尋找特定憑證。

搜尋 COA

已經擁有該產品？

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

客戶也查看了

Slide 1 of 3

1 of 3

Protein Kit photometric, 0.5-10 g/L (Protein), Spectroquant®

Supelco

1.10307

Protein Kit

Lowry试剂

Supelco

L3540

Lowry试剂

Sigma-Aldrich

Sigma-Aldrich

57697

Advanced Protein Assay

Sook Hyun Chung et al.
Scientific reports, 6, 28993-28993 (2016-07-05)
Vascular changes and photoreceptor degeneration are features of age-related macular degeneration, diabetic retinopathy and macular telangiectasis. We have profiled the differential expression of microRNAs and analysed their target genes in transgenic mice in which induced Müller cell disruption results in
Eneida V Reis et al.
Parasitology international, 63(1), 120-126 (2013-10-24)
Vaults are ribonucleoproteins (13 MDa) highly conserved among lower and higher eukaryotes. Their association produces a complex composed of three proteins named Major Vault Protein (MVP), vault (PolyADP-ribose) polymerase (VPARP) and Telomerase-associated protein (TEP1), plus a small untranslated RNA. The
A new strategy for battling bacterial resistance: Turning potent, non-selective and potentially non-resistance-inducing biocides into selective ones
Ghanbar S, et al.
Nanomedicine: Nanotechnology, Biology, and Medicine (2018)
Jingwei Zhang et al.
Acta biomaterialia, 10(7), 3254-3263 (2014-04-01)
The microporosity of calcium phosphate (CaP) ceramics has been shown to have an essential role in osteoinduction by CaP ceramics after ectopic implantation. Here we show that it is not the microporosity but the size of surface microstructural features that
PPAR-? agonist GL516 reduces oxidative stress and apoptosis occurrence in a rat astrocyte cell line
Neurochemistry International (2019)
查看所有相關論文

文章

Cell Lysate Preparation

Cell lysis challenges and protein extraction variables for Western blot sensitivity and reproducibility.

Universal Proteomics Standards

High-throughput proteomics advances with improved analysis methods and mass spectrometry.

Western Blot Analysis of Immunoprecipitation (IP-Western)

The possible causes and potential remedies for challenges encountered in the immunoprecipitation-Western blot technique, which consists of cell lysis, formation of the antibody-antigen (immune) complex, precipitation of the immune complexes, and analysis by Western blotting.

免疫沉澱的 Western 印迹分析 (IP-Western)

免疫沉澱-Western 印跡技術（包括細胞裂解、形成抗體-抗原（免疫）複合物、免疫複合物沉澱以及 Western 印跡分析）中遇到的挑戰的可能原因和潛在補救方法。

條款

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

相關內容

Protein Quantitation

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Tools for Protein Quantitation

Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

蛋白質定量

蛋白質定量方法、試劑和免疫分析技術，用於準確測量各種樣品中的蛋白質濃度。

Questions

1–2 of 2 Questions  

  1. How many samples (1 mL) can be analysed with the kit?

    1 answer

    1. Below is the link to the product bulletin. The reagents and their volumes included in the kit can be found on page 1. Table 1 on page 2 shows the volume of QuantiPro Working Reagent to prepare and the amount of each component needed:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/358/950/qpbcabul.pdf

      For 15 2 ml Standard Test tube assays, 7.75 ml of QA, 7.75 ml of QB, and 0.31 of Copper(II) Sulfate Reagent QC are needed. By calculation, about 480 assays can be done. Fewer may be gotten due to loss during pipetting. Also one may use more or less Protein Standard Solution depending upon how many assays they do each time. Protein Standard Solution (P0914) is available separately in case one needs more for their assays.

      Helpful?

  2. How many samples can be analyzed for one kit ? How long can the reagents be kept and at which temperature ?

    1 answer

    1. This kit can be used in several different formats. The format chosen, the number of blanks, and standards will determine the number of tests. The protein standard requires refrigerated storage. Other components are stable up to 1 week at room temperature. For full storage details and test count information, please refer to the technical bulletin, page 2. Click on the link below to review this document:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/358/950/qpbcabul.pdf

      Helpful?

Reviews

No rating value

Active Filters

我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務