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Merck
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重要文件

TP0100

Sigma-Aldrich

总蛋白试剂盒,微量

同義詞:

Bradford

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.32
暫時無法取得訂價和供貨情況

描述

wavelength 595 nm
total test time 2 min
working reagent stability 18-26°C for 2 weeks
dynamic range 50 mg/dL 0.5 g/L

參數

18-26 °C temperature
50 μL sample volume

儲存溫度

2-8°C

一般說明

蛋白质测定是生化研究最常见的实验操作之一。微量总蛋白试剂盒提供了一种简单、快速、高灵敏度的方法,只需 50μ L 或更少的样品。

應用

微量总蛋白试剂盒已被用于测定:
  • 通过Bradford 法测定细菌匀浆液中的总蛋白[1]
  • 通过Bradford 微量检测法测定组织提取物中的总蛋白含量[2]
  • 血清总蛋白[3]
  • 通过Bradford 法测定叶片中的可溶性蛋白质 [4]

生化/生理作用

微量总蛋白试剂盒可用于测定溶液中蛋白质的浓度。

適合性

可测定多达0.5mg/ml 的蛋白样品浓度。

原則

亮蓝G-250(考马斯亮蓝)在酸性条件下,与蛋白质反应,形成蓝色蛋白质染料复合物。该复合物在595nm处的吸光度与蛋白质含量成正比。

測試方式

比色法,终点法(Endpoint)

套裝中的組件也可單獨購買

產品號碼
描述
SDS

  • Protein Standard 5 mL

訊號詞

Danger

危險分類

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1

標靶器官

Eyes,Central nervous system

儲存類別代碼

3 - Flammable liquids

閃點(°F)

132.8 °F

閃點(°C)

56 °C


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