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Merck
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B6916

Supelco

Bradford试剂

for 0.1-1.4 mg/ml protein

同義詞:

考马斯染料结合蛋白检测,蛋白染料试剂

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About This Item

分類程式碼代碼:
12161500
NACRES:
NA.32

品質等級

形狀

solution

儲存溫度

2-8°C

相關類別

一般說明

Bradford检测是把考马斯亮蓝G-250加入至蛋白溶液中。考马斯蓝染料可与碱性和芳香族氨基酸结合,从而在蛋白质测定过程中引起吸光值的迁移。

應用

Bradford试剂已用于测定总蛋白的浓度。

特點和優勢

  • 该试剂为即用型。 无需混合或稀释。
  • 显色很快速。 仅需五分钟的孵育便能将样本在595 nm处进行读值。
  • 带有硫醇的还原性糖和还原性底物并不会干扰该试剂。
  • 试剂适用于微量(1-10 μg/ml)及标准(50-1400 μg/ml)的检测。
  • 可用于微孔板检测。
  • 低成本检测。

法律資訊

應用

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描述
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象形圖

Health hazardCorrosion

訊號詞

Warning

危險分類

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2

標靶器官

Eyes,Central nervous system

儲存類別代碼

8B - Non-combustible corrosive hazardous materials

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Sin-Jin Li et al.
Clinical nutrition (Edinburgh, Scotland), 36(3), 760-767 (2016-06-28)
The cellular mechanisms of obesity-induced cardiomyopathy are multiple and not completely elucidated. The objective of this study was to differentiate two obesity-associated cardiomyopathy miniature pig models: one with the metabolic syndrome (MetS), and one with a metabolically healthy obesity (MHO).
Influence of condensing equipment and temperature on exhaled breath condensate pH, total protein and leukotriene concentrations.
Czebe K et al.
Respiratory Medicine, 102, 720-720 (2008)
M Tal et al.
The Journal of biological chemistry, 260(18), 9976-9980 (1985-08-25)
Dimethyl sulfoxide was found to be effective for extraction of Coomassie Brilliant Blue R-250 (Coomassie R) from stained proteins on polyacrylamide gel slices. A good correlation was found between the ability of different proteins to bind Coomassie R and their
Role of rpoS in the development of cell envelope resilience and pressure resistance in stationary-phase Escherichia coli.
Charoenwong D et al.
Applied and Environmental Microbiology, 77, 5220-5229 (2011)
Hugh S Winwood-Smith et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 313(4), R347-R356 (2017-07-14)
Long-term studies have found that low-carbohydrate diets are more effective for weight loss than calorie-restricted diets in the short term but equally or only marginally more effective in the long term. Low-carbohydrate diets have been linked to reduced glycogen stores

文章

The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.

本討論將強調其中一些方法,即使用多重反應監測 (MRM) 和 Protein-AQUA。

條款

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.

從細胞培養和組織樣本中製備 Western blot 樣本的規則和良好實踐。

相關內容

蛋白質定量方法、試劑和免疫分析技術,用於準確測量各種樣品中的蛋白質濃度。

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

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