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重要文件

P8169

Sigma-Aldrich

Phytagel

Phytagel™

BioReagent, suitable for plant cell culture, powder

同義詞:

植物凝胶, 琼脂替代品胶凝剂

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About This Item

CAS號碼:
EC號碼:
MDL號碼:
分類程式碼代碼:
10171502
NACRES:
NA.72
暫時無法取得訂價和供貨情況

產品線

BioReagent

形狀

powder

技術

cell culture | plant: suitable

應用

agriculture

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一般說明

常见工作浓度:在植物组织培养基中1.5-2.5 g/L;在微生物培养基中最高10 g/L。Phytagel需要阳离子(特别是二价)用于凝胶的发生。大多数植物组织培养基中通常含有的钙和镁浓度足以实现凝胶化。低盐培养基配方,特别是那些用于微生物应用的可能需要补充额外的钙或镁盐(如CaCl2或MgSO4)或更高浓度的Phytagel。

應用

Phytagel已用于:
  • 拟南芥中根检测的培养基固化[1]
  • 作为农杆菌培养中固体Paul′s培养基的一种成分[2]
  • 冬青属组织培养中根诱导培养基的一种组分[3]

生化/生理作用

Phytagel旨在用于代替琼脂和其他用于植物组织培养的凝胶剂。Phytagel已被证明是香蕉幼苗微繁殖中组织培养级琼脂的优质替代品。

其他說明

一种由葡萄糖醛酸、鼠李糖和葡萄糖组成的细菌基质产生的琼脂替代品. 它能产生透明、无色、高强度的凝胶,有助于微生物污染的检测。

重構

为防止结块,应在加热前缓慢的加入到快速搅拌的培养基中。

法律資訊

Phytagel is a trademark of Sigma-Aldrich Co. LLC

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儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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存取文件庫

Christine Le Roux et al.
Applied and environmental microbiology, 75(24), 7752-7759 (2009-10-27)
In the context of an increasing utilization of the interspecific hybrid Acacia mangium x A. auriculiformis as a plantation tree in the tropical humid zone, its symbiotic characterization was carried out in comparison with that of its two parental species.
Abhishek Bhaskaran et al.
Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology, 19(5), 874-880 (2016-05-22)
Longer procedural time is associated with complications in radiofrequency atrial fibrillation ablation. We sought to reduce ablation time and thereby potentially reduce complications. The aim was to compare the dimensions and complications of 40 W/30 s setting to that of
Micropropagation of Ilex dumosa (Aquifoliaceae) from nodal segments in a tissue culture system
Luna C, et al.
Biocell, 27(2), 205-212 (2003)
CEP genes regulate root and shoot development in response to environmental cues and are specific to seed plants
Delay C, et al.
Journal of Experimental Botany, 64(17), 5383-5394 (2013)
Cytoskeletal dynamics in interphase, mitosis and cytokinesis analysed through Agrobacterium-mediated transient transformation of tobacco BY-2 cells
Buschmann H, et al.
The New phytologist, 190(1), 258-267 (2011)

條款

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

植物組織培養中使用抗生素和抗真菌劑的參考指南和製備指南。

Questions

1–3 of 3 Questions  
  1. Buongiorno. Vorrei sapere se il Phytagel è Gellano ad alto o a basso grado di acilazione

    1 answer
    1. This product is characterized as having a low acyl content.

      Helpful?

  2. What is the molecular weight of one repeating unit?

    1 answer
    1. The molecular weight is not determined. However, the constituent sugars of Phytagel are glucose, glucuronic acid and rhamnose in the molar ratio of 2:1:1.

      Helpful?

  3. What is the gelling strength of Phytagel P8169

    1 answer
    1. The Gel Strength for Phytagel (P8169) is 700 g/cm2 (tested at 0.5%).

      Helpful?

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