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重要文件

G1910

Sigma-Aldrich

Gelzan CM 结冷胶

Gelrite®

同義詞:

植物凝胶, 琼脂替代品胶凝剂

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About This Item

CAS號碼:
EC號碼:
MDL號碼:
分類程式碼代碼:
10171502
NACRES:
NA.72
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品質等級

應用

agriculture

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一般說明

结冷胶,吉兰糖胶,可替代琼脂用作微生物和组织培养基中的凝胶剂。结冷胶广泛用于各种固定基质。在电解液的存在下,可以低浓度形成坚实的凝胶。结冷胶是由2:1:1的D-葡萄糖、L-鼠李糖和D-葡糖醛酸经β(1→4)糖苷键连接而成的线性四糖。
结冷胶,吉兰糖胶,可替代琼脂用作微生物和组织培养基中的凝胶剂。结冷胶广泛用于各种固定基质。在电解液的存在下,可以低浓度形成坚实的凝胶。结冷胶是由2:1:1的D-葡萄糖、L-鼠李糖和D-葡糖醛酸经β (1→4)糖苷键连接而成的线性四糖。

應用

Gelzan CM结冷胶已用于:
  • 结冷胶-普鲁兰多糖纳米凝胶的化学交联合成
  • 结冷胶-普鲁兰多糖水凝胶合成
  • 加入KNOP培养基,维持和制备角苔(Anthoceros agrestis)叶状体组织样品进行成像

Gelzan CM结冷胶已用于:
  • 结冷胶-普鲁兰多糖纳米凝胶的化学交联合成[1]
  • 结冷胶-普鲁兰多糖水凝胶合成[2]
  • 加入KNOP培养基,维持和制备角苔(Anthoceros agrestis)叶状体组织样品进行成像[3]

结冷胶,吉兰糖胶,被推荐用作微生物培养基中琼脂的凝胶剂。 吉兰糖胶被广泛应用于各种固定基质中。

特點和優勢

  • 透明和无色的琼脂替代品
  • 机械强度高
  • 在水中轻松分散
  • 低粘度
  • 融胶温度高

法律資訊

Gelrite is a registered trademark of CP Kelco U.S., Inc.
Gelzan is a trademark of CP Kelco U.S., Inc.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

Lot/Batch Number

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存取文件庫

Timothy E L Douglas et al.
Biomedical materials (Bristol, England), 12(2), 025015-025015 (2017-02-23)
Injectable composites for tissue regeneration can be developed by dispersion of inorganic microparticles and cells in a hydrogel phase. In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum
Timothy E L Douglas et al.
Journal of biomedical materials research. Part A, 106(3), 822-828 (2017-10-24)
Mineralized hydrogels are increasingly gaining attention as biomaterials for bone regeneration. The most common mineralization strategy has been addition of preformed inorganic particles during hydrogel formation. This maintains injectability. One common form of bone cement is formed by mixing particles
Gustavo de Medeiros et al.
Nature communications, 6, 8881-8881 (2015-11-26)
Selective-plane illumination microscopy has proven to be a powerful imaging technique due to its unsurpassed acquisition speed and gentle optical sectioning. However, even in the case of multiview imaging techniques that illuminate and image the sample from multiple directions, light
Marco A Lopez-Heredia et al.
Journal of tissue engineering and regenerative medicine, 12(8), 1825-1834 (2018-04-28)
Mineralization of hydrogels is desirable prior to applications in bone regeneration. CaCO3 is a widely used bone regeneration material, and Mg, when used as a component of calcium phosphate biomaterials, has promoted bone-forming cell adhesion and proliferation and bone regeneration.
Timothy E L Douglas et al.
Journal of tissue engineering and regenerative medicine, 11(5), 1610-1618 (2015-07-16)
Gellan gum hydrogels functionalized with alkaline phosphatase were enzymatically mineralized with phosphates in mineralization medium containing calcium (Ca) and zinc (Zn) to improve their suitability as biomaterials for bone regeneration. The aims of the study were to endow mineralized hydrogels

條款

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

植物組織培養中使用抗生素和抗真菌劑的參考指南和製備指南。

Questions

1–10 of 11 Questions  
  1. Does Gellan Gum/Gelrite have Iron, Manganese and Zinc in trace amount? It would be great to have the specific concentration detected. Thank you.

    1 answer
    1. Unfortunately, the levels of Iron, Manganese and Zinc are not analyzed for this product.

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  2. How many grams per liter of Product G1910, Gelzan™ CM are recommended for plant tissue culture?

    1 answer
    1. Gelzan™ CM is typically used at a concentration of 2-4 grams per liter.

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  3. Buonasera, sarebbe possibile sapere il grado di purezza del Gelzan? visto che nel Gelzan ci sarebbero sali o altri componenti, quanto è la concentrazione del Gellan Gum nel Gelzan? Rimango in attesa di un vostro riscontro.

    1 answer
    1. The purity of this gelling agent is not determined. This material is a complex polysaccharide comprised of glucose, rhamnose, and glucuronic acid in the approximate molar ratio of 2:1:1. In addition to the base components, historical ICP analysis shows trace amounts of Calcium, Magnesium, Potassium, Phosphate, and Sodium. The maximum moisture content for this product is 16%. Please see the link below to review a sample Certificate of Analysis:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/G1910-BULK0000254218.pdf

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  4. What is the difference between Product G1910, Gelzan CM and Gelrite?

    1 answer
    1. The products are chemically the same. However, Gelzan CM is produced through a new and more efficient process. There are some slight differences in the specifications of the two products.

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  5. What is the gel strength of Product G1910, Gelzan™ CM?

    1 answer
    1. The gel strength as determined by the vendor is between 400-700 g/cm2.

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  6. How do I use Product G1910, Gelzan™ CM?

    1 answer
    1. The G1910 is added to well-stirred water, and the dispersion heated to 85-90°C to ensure full hydration. To form a gel, the necessary amount of electrolyte, such as potassium citrate or calcium chloride, is added to the solution at 85°C and the mixture is poured or pumped into containers and allowed to cool undisturbed. The product is biodegradable, so a biocide is recommended. The typical use level is 0.2 to 2.0%.

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  7. Is Product G1910, Gelzan™ CM, different that Gelrite?

    1 answer
    1. The G1910 is chemically the same as Gelrite, which is no longer produced. Both items are trademarked to CP Kelco U.S., Inc. They are both Gellan Gum, but the manufacturer has different specifications for these items.

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  8. Does Product G1910, Gelzan CM, have an expiration date?

    1 answer
    1. The lots of this product are not assigned expiration dates. The guarantee of quality is one year from the time of delivery for unopened bottles stored at room temperature.

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  9. What is the particle size of Product G1910, Gelzan™ CM?

    1 answer
    1. The vendor supplied particle size is 42 mesh, or about 355 μm, with a specification of not less than 95% through. This means that at least 95% of the particles should be smaller than 355 microns in diameter.

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  10. What is Product G1910, Gelzan CM?

    1 answer
    1. It is a low-acyl gellan gum used as a gelling agent in both microbiological assay media and tissue culture.

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1–10 of 11 Questions  

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