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M5519

Sigma-Aldrich

Murashige & Skoog 基本培养基

powder, suitable for plant cell culture

同義詞:

MS 基本培养基

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About This Item

MDL號碼:
MFCD00240976
分類程式碼代碼:
12352207
NACRES:
NA.72
暫時無法取得訂價和供貨情況

形狀

powder

技術

cell culture | plant: suitable

應用

agriculture

儲存溫度

2-8°C

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相關類別

一般說明

Murashige & Skoog培养基广泛用于植物组织生长培养基。 M&S基础培养基含有大量营养素，包括高含量的硝酸盐和有机添加剂，如琼脂、糖、维生素和生长调节剂。 经常添加到M&S中的重要生长调节剂包括IAA（生长素/形态发生素）和激动素（细胞分裂素/细胞分裂促进剂）。

應用

Murashige & Skoog培养基可用于转化研究用植物组织培养基。[1][2]

公式變體

含有Linsmaier 和 Skoog（1962）所述的大量、微量营养素和维生素。

培养基配方

數量

配制成每升培养基含 4.4 克粉末。

準備報告

Murashige & Skoog培养基可以由粉末复溶，或者将作为M&S完全培养基主要成分（大量营养素混合物和维生素混合物）的产品混合后复溶。Murashige & Skoog培养基（M5519）包含原始经典配方中的微量营养素和维生素。 它可以添加蔗糖、琼脂、生长素（IAA）和细胞分裂素（激动素），形成植物组织生长完全培养基。

相關產品

產品號碼
描述
訂價

象形圖

Flame over circleExclamation mark
GHS03,GHS07

訊號詞

Warning

危險聲明

H272,H319

危險分類

Eye Irrit. 2 - Ox. Sol. 3

儲存類別代碼

5.1B - Oxidizing hazardous materials

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
0000422941
0000422941
0000366116
0000366116
0000388916

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Ajay Kumar et al.
BMC genomics, 13, 597-597 (2012-11-07)
Development of a high quality reference sequence is a daunting task in crops like wheat with large (~17Gb), highly repetitive (>80%) and polyploid genome. To achieve complete sequence assembly of such genomes, development of a high quality physical map is
Joan E Krochko et al.
Methods in molecular biology (Clifton, N.J.), 773, 113-134 (2011-09-08)
In vitro assays for cytochrome P450 enzymes developed from plant-derived microsomal extracts have not been used extensively for the characterization and quantification of enzyme activities in plant tissues. We describe here an in vitro assay for abscisic acid (ABA) 8'-hydroxylase
Youjun Zhang et al.
Plant communications, 1(5), 100028-100028 (2020-12-29)
Although the use of stable transformation technology has led to great insight into gene function, its application in high-throughput studies remains arduous. Agro-infiltration have been widely used in species such as Nicotiana benthamiana for the rapid detection of gene expression and
Thierry G A Lonhienne et al.
Scientific reports, 4, 5055-5055 (2014-05-24)
Growth, morphogenesis and function of roots are influenced by the concentration and form of nutrients present in soils, including low molecular mass inorganic N (IN, ammonium, nitrate) and organic N (ON, e.g. amino acids). Proteins, ON of high molecular mass
Liang Zhu Y et al.
Plant physiology, 119(1), 73-80 (1999-01-08)
An important pathway by which plants detoxify heavy metals is through sequestration with heavy-metal-binding peptides called phytochelatins or their precursor, glutathione. To identify limiting factors for heavy-metal accumulation and tolerance, and to develop transgenic plants with an increased capacity to
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Questions

1–10 of 11 Questions  

  1. How do I prepare this medium?

    1 answer

    1. The preparation instructions for this product are as follows:
      1. Using a container twice the size of the desired final volume, measure out ~90% of the required final volume of tissue culture grade water (e.g., Catalog Number W3500). Example: 900 ml for a final volume of 1000 ml.
      2. While stirring, add the powdered product.
      3. Rinse the original container with a small volume of tissue culture grade water to remove traces of the powder. Add to the solution in step 2.
      4. Add desired supplements (e.g., sucrose, gelling agent, auxins, cytokinins).
      Add heat labile constituents after autoclaving.
      5. While stirring, adjust to the desired pH (e.g., 5.7±0.1) using KOH, NaOH, or HCl.
      6. Add additional tissue culture grade water to bring the medium to the final volume.
      7. If a gelling agent is used, heat the solution to clarity while stirring.
      8. Dispense the medium into culture vessels before or after autoclaving according to the application.
      Add heat labile constituents after autoclaving.
      9. Sterilize the medium in a validated autoclave at 1 kg/cm2 (15 psi). The medium should attain a temperature of 121 °C for at least 15 minutes.

      Please see the link below to review this and additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf

      Helpful?

  2. What is the difference between Product M5519, Murashige and Skoog (MS) Basal Medium and M5524, Murashige and Skoog Basal Salt Mixture?

    1 answer

    1. Product M5524 is the Murashige and Skoog(M&S) Basal Salt Mixture, only. The M5519 products is M&S Basal Medium which also includes the vitamins myo-Inositol, Nicotinic acid and Pyridoxine HCl. These formulations are identical with the exception of vitamin components. With the addition of 1 mL of M7150 M&S Vitamin Solution, the composition of each medium is equivalent.

      Helpful?

  3. What is the formulation of Product M5519, Murashige and Skoog (MS) Basal Medium?

    1 answer

    1. Please see the link below to review the product datasheet, including the full formulation:

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf

      To review additional Murashige and Skoog formulations, please see the link below:

      https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/plant-tissue-culture/murashige-skoog

      Helpful?

  4. Product M5519, Murashige and Skoog Basal Medium, is on back order. Can you suggest an alternate?

    1 answer

    1. Product M5524 Murashige and Skoog(M&S) Basal Salt Mixture, is an ideal alternative to M5519, M&S Basal Medium. These formulations are identical with the exception of vitamin components. With the addition of 1 mL of M7150 M&S Vitamin Solution, the composition of each medium is equivalent.

      Helpful?

  5. Can I autoclave Product M5519, Murashige and Skoog Basal Medium?

    1 answer

    1. Yes, this medium is autoclavable.

      Helpful?

  6. once the media is prepared, what conditions should it be stored in, and how long can it be stored?

    1 answer

    1. The prepared solution is quite stable but should be stored refrigerated. Monitor the salt solution for particulates or turbidity. After the addition of agar and subsequent sterilization, poured plates or vessels should also be stored aseptically in the refrigerator until use. The shelf life of the stored plates or vessels is most often limited by less stable compounds added to the medium. The end user would have to determine the stability of these components. While there is no firm shelf life on the prepared medium, contamination, and drying are the most common reasons for degradation. Monitor plates/vessels for contaminants or turbidity. An indication of excessive drying will be the gelled medium pulling away from the sides of the plate or vessel. The medium has lost moisture and may not be suitable for use. Moisture loss can be minimized by storing prepared plates/vessels in a sealed container or sealed bags. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf

      Helpful?

  7. What is the percentage of sucrose in M5519?

    1 answer

    1. The M5519 does not contain sucrose. This component would have to be added prior to autoclaving. The typical amount is 30 g/L. See the link below to review the product information sheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf

      Should sucrose be desired, see product M9274, which is more of a complete formulation containing M&S salts, vitamins, sucrose, and agar. See the link below to review the formulations of the various Murashige & Skoog media:
      https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/plant-tissue-culture/murashige-skoog#Basal%20Salts%20Mixtures

      Helpful?

  8. What is the quantity and what is shelf life

    1 answer

    1. The SKU sizes indicated refer to the final volume of media that can be made upon reconstitution of the pre-weighted powder. This powdered media is formulated to contain 4.4 grams of powder per liter of medium. This product does not contain an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services

      Helpful?

  9. How much agar is there in Product M5519, Murashige and Skoog Basal Medium?

    1 answer

    1. This product does not contain any agar.

      Helpful?

  10. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

1–10 of 11 Questions  

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