跳轉至內容
Merck
全部照片(9)

重要文件

A6013

Sigma-Aldrich

琼脂糖

Agarose

Type I, low EEO

同義詞:

3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose LE

登入查看組織和合約定價


About This Item

CAS號碼:
EC號碼:
MDL號碼:
分類程式碼代碼:
41105317
PubChem物質ID:
NACRES:
NA.25
暫時無法取得訂價和供貨情況

生物源

algae (Gelidium & Gracilaria)

品質等級

種類

Type I

形狀

powder

技術

electrophoresis: suitable

雜質

≤10% water

EEO

0.09-0.13

轉變溫度

gel point 36 °C ±1.5 °C (1.5% gel)

凝膠強度

≥1200 g/cm2 (1% gel)

負離子痕跡

sulfate (SO42-): ≤0.15%

適合性

suitable for electrophoresis

SMILES 字串

O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI 密鑰

MJQHZNBUODTQTK-WKGBVCLCSA-N

尋找類似的產品? 前往 產品比較指南

一般說明

琼脂是从红藻、石花菜江蓠中提取的高分子聚合物,常用于核酸分离。I型琼脂糖在分子生物学中常用于制备电泳凝胶,凝胶电泳技术可根据分子大小分离和分析DNA或RNA片段或蛋白。在植物细胞培养中,琼脂糖还可用于制作水凝胶,一种提供三维培养基质的凝胶样材料。为植物细胞提供支持和生物相容性环境,这种模拟生理条件可用于进行体内实验。在植物生物学中应用广泛,包括细胞生长、组织培养研究,以及分析植物对不同刺激的应答反应。

應用

1型琼脂糖常用于分子生物学:
  • DNA/RNA分子的凝胶电泳[1][2]
  • 作为细胞培养的琼脂糖覆盖物,用于放射扩散法测定抗菌活性[3]
  • 作为细胞培养的琼脂糖覆盖物,用于放射免疫扩散法测定抗原[4]
  • 作为琼脂糖覆盖物,用于蚀斑试验测定病毒滴度
  • 制备琼脂糖水凝胶包封粘性介质用于三维细胞培养[5]
  • 人组织样MRI体模组分[6]
  • 脉冲场凝胶电泳法分析患者样本MRSA基因组用于诊断[2]

特點和優勢

  • 低电渗(Low EEO)琼脂糖适用于一般的核酸分析
  • 适用电泳:不含DNA酶&RNA酶
  • 适用植物细胞培养
  • 适用哺乳动物细胞培养

分析報告

以下是与我们的琼脂糖相关的性质列表:
硫酸盐含量 - 用作纯度指标,因为硫酸盐是其中存在的主要离子基团。
凝胶强度 - 破裂凝胶所需施加的力。
胶凝点 - 琼脂糖水溶液在冷却时的胶凝温度。 琼脂糖溶液在液体-凝胶转变的过程中会表现出滞后现象 - 也就是说,它们的凝胶点与它们的熔点温度不同。
电内渗(EEO)- 液体通过凝胶的一种运动。琼脂糖凝胶中的阴离子基团被固定在基质上不能移动,但可解离的抗衡阳离子可以向基质中的阴极迁移,从而产生EEO。 由于生物聚合物的电泳运动通常是朝向阳极的,因此EEO会因为内部的对流而破坏分离。

其他說明

如需了解生化试剂系列的更多信息,请填写此表

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


從最近期的版本中選擇一個:

分析證明 (COA)

Lot/Batch Number

未看到正確版本?

如果您需要一個特定的版本,您可以透過批號來尋找特定憑證。

已經擁有該產品?

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

3D culture of bovine articular chondrocytes in viscous medium encapsulated in agarose hydrogels for investigation of viscosity influence on cell functions
Zheng J et al.
Journal of materials chemistry. B, 11, 7424-7434 (2023)
Comparative study on characteristics of lysozymes from the hemolymph of three lepidopteran larvae, Galleria mellonella, Bombyx mori, Agrius convolvuli
Yu KH et al.
Developmental and Comparative Immunology, 26, 707-713 (2002)
Whispering gallery mode micro resonators for multi-parameter sensing applications
Mallik AK, et al.
Optics Express, 26(24), 31829-31838 (2018)
Intrahepatic hepatitis C RNA levels do not correlate with degree of liver injury in patients with chronic hepatitis C
McGuinness P H et al.
Hepatology, 23, 676-687 (1996)
In Vivo Superresolution Imaging of Neuronal Structure in the Mouse Brain
Urban BE, et al.
IEEE Transactions on Bio-Medical Engineering, 65(1), 232-238 (2018)

Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the molecular weight of agarose (Product No- A6013)?

    1 answer
    1. The molecular weight for product A6013 has not been determined. However, the typical molecular weight for agarose is approximately 630.5 g/mol, with a formula of C12H20O10(C12H18O9)n.

      Helpful?

Reviews

Active Filters

  1. 1 Ratings-Only Review

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務