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重要文件

P4439

Sigma-Aldrich

碱性磷酸酶 来源于猪肾脏

lyophilized powder, ≥100 DEA units/mg protein

同義詞:

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
eCl@ss:
42010105
NACRES:
NA.54
暫時無法取得訂價和供貨情況

形狀

lyophilized powder

比活性

≥100 DEA units/mg protein

成份

Protein, ≥50%

儲存溫度

−20°C

應用

碱性磷酸酶可与抗体和其他蛋白质偶联,用于ELISA、蛋白质免疫印迹和组织化学检测。 碱性磷酸酶也可用于防止DNA自身连接和放射性标记[1]

生化/生理作用

碱性磷酸酶是一种水解酶,能使核苷酸、蛋白质和生物碱等分子去磷酸化。

包裝

包装规格取决于DEA单位

單位定義

在pH 9.8和37℃条件下,1.0个单元每分钟水解1 μmol对硝基苯基磷酸盐。

外觀

从Tris-HCl缓冲液中冻干,含氯化镁和氯化锌,pH 8.0

分析報告

通过缩二脲法测定的蛋白质。

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Sequencing end-labeled DNA with base-specific chemical cleavages.
A M Maxam et al.
Methods in enzymology, 65(1), 499-560 (1980-01-01)
Naomi Ofer et al.
The Journal of biological chemistry, 288(16), 11287-11293 (2013-03-09)
Protein degradation via prokaryotic ubiquitin-like protein (Pup) tagging is conserved in bacteria belonging to the phyla Actinobacteria and Nitrospira. The physiological role of this novel proteolytic pathway is not yet clear, although in Mycobacterium tuberculosis, the world's most threatening bacterial
Gouri Chaudhuri et al.
Indian journal of biochemistry & biophysics, 50(1), 64-71 (2013-04-27)
The hydrolysis of p-nitrophenyl phosphate (pNPP) by calf intestinal alkaline phosphatase (CIAP) was investigated with respect to kinetic parameters such as V(max), K(m) and K(cat) under varying pH, buffers, substrate concentration, temperature and period of incubation. Highest activity was obtained
Lars Hansen et al.
American journal of human genetics, 92(4), 575-583 (2013-04-09)
PGAP2 encodes a protein involved in remodeling the glycosylphosphatidylinositol (GPI) anchor in the Golgi apparatus. After synthesis in the endoplasmic reticulum (ER), GPI anchors are transferred to the proteins and are remodeled while transported through the Golgi to the cell
Insulin affects dopamine metabolism in Drosophila females under normal and stress conditions.
N V Adonyeva et al.
Doklady. Biochemistry and biophysics, 448, 40-42 (2013-03-13)

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Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

碱性磷酸酶的酶法测定,二乙醇胺法(EC 3.1.3.1)

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