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重要文件

M3168

Sigma-Aldrich

4-Methylumbelliferyl phosphate

alkaline phosphatase substrate, liquid

同義詞:

4-methylumbelliferone phosphate Liquid Substrate System

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About This Item

CAS號碼:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
PubChem物質ID:
NACRES:
NA.32
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產品名稱

4-甲基伞形酮磷酸酯 (4-MUP) 液体底物系统, liquid

形狀

liquid

儲存溫度

2-8°C

SMILES 字串

CC1=CC(=O)Oc2cc(OP(O)(O)=O)ccc12

InChI

1S/C10H9O6P/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14/h2-5H,1H3,(H2,12,13,14)

InChI 密鑰

BCHIXGBGRHLSBE-UHFFFAOYSA-N

相關類別

一般說明

4-甲基伞形酮磷酸酯 (4-MUP) 是碱性磷酸酶的底物,碱性磷酸酶形成可溶性荧光物质甲基伞形酮。4-MUP 液体底物系统在即用型试剂中结合了 4-MUP 和缓冲液。

應用

4-甲基伞形磷酸酯(4-MUP)液体底物系统已被用作酶联免疫吸附测定(ELISA)中碱性磷酸盐的荧光底物。[1][2][3]它也已被用于分泌的胚胎碱性磷酸酶(SEAP)活性测定中。[4]

分析報告

4-MUP 荧光可在 360 nm 激发和 440 nm 发射下测量。也可使用紫外光源观察荧光终产物。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Larre C, et al.
Journal of Proteomics, 74(8), 1279-1289 (2011)
Modification of pasta structure induced by high drying temperatures. Effects on the in vitro digestibility of protein and starch fractions and the potential allergenicity of protein hydrolysates
Petitot M, et al.
Food Chemistry, 116(2), 401-412 (2009)
Activation of NF-kappaB by rank requires tumor necrosis factor receptor-associated factor (TRAF) 6 and NF-kappaB-inducing kinase identification of a novel TRAF6 interaction motif
Darnay BG, et al.
The Journal of biological chemistry, 274(12), 7724-7731 (1999)
Degree of hydrolysis and antigenicity of buffalo alpha S1 casein and its hydrolysates in children with cow milk allergy
Ahmad N, et al.
Food and agricultural immunology, 27(1), 87-98 (2016)
Christopher P Holmes et al.
Bioorganic & medicinal chemistry letters, 18(11), 3382-3385 (2008-04-30)
As part of a program to develop better PTP1B fluorogenic substrates that more closely mimic the functionality found in the natural substrate, we have prepared and evaluated nine novel analogs of 4-methylumbelliferone phosphate (MUP) with a variety of additional groups

Questions

  1. Which stop solution is the best option for AP-4MUP ELISA? Please advise.

    1 answer
    1. The M3168 product does not require a stop solution. 4MUP is a fluorescent substrate. Adding a stop solution to 4MUP, such as an acidic solution like 2M Sulfuric Acid, would greatly reduce or eliminate the fluorescence. While stop solutions are typically added to substrates like pNPP, TMB, or OPD, they are not required when working with product M3168. The Product Information Sheet for M3168 can be downloaded from the website, where it mentions that the solution is ready to use and nothing else should be added to it.

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