跳轉至內容
Merck
全部照片(4)

文件

NA2020

Sigma-Aldrich

GenElute™ 血液基因组DNA试剂盒

sufficient for 350 purifications

同義詞:

血液基因组DNA, Gen Elute

登入查看組織和合約定價
全部照片(4)

About This Item

分類程式碼代碼:
41105501
NACRES:
NA.52

用途

sufficient for 350 purifications

品質等級

200

技術

DNA purification: suitable

儲存溫度

15-25°C
room temp

尋找類似的產品？ 前往 產品比較指南

相關類別

一般說明

GenElute血液基因组DNA试剂盒可以简单、方便地从新鲜或长时间存放（超过24小时）的全血中分离高纯度基因组DNA。该试剂盒将硅胶膜技术的优势与微量离心形式结合在一起，同时无需采用昂贵的树脂、乙醇沉淀，以及诸如苯酚和氯仿等有害化学成分。

應用

纯化所得的基因组DNA可用于以下下游应用：
  • 限制性内切酶酶切
  • PCR
  • Southern印迹
  • 测序反应
  • 克隆

特點和優勢

  • 起始材料：不超过200 μl新鲜或长时间存放的血液
  • 预期得率：至多10 μg
  • 洗脱体积：400 μl
  • 所需时间：<40分钟
  • A260/A280比率：1.6 - 1.9
  • 兼容多种抗凝血素、包括EDTA、肝素和柠檬酸钠

原則

起始材料首先溶解在含有离液盐的溶液之中，以确保大分子彻底变性。之后加入乙醇，从而使DNA在溶解物通过微量离心管在硅胶膜上离心时，结合到膜上。随附一份Prewash Solution，可帮助与全血样本长时间存放（超过24小时）相关的污染物。在洗涤去除污染物后，将DNA放在200 mL的Tris-EDTA溶液中洗脱。

预期基因组DNA得率取决于所用的起始材料的数量和性质（例如，从200 μl 新鲜的全血之中，可在一小时内分离出4至10 μg RNase A处理的DNA）。通过本试剂盒纯化所得的DNA的A260/A280比率介于1.6-1.9之间，最大长度可达50 kb。

其他說明

更多信息，请见www.sigma-aldrich.com/genomicdna。

法律資訊

GenElute is a trademark of Sigma-Aldrich Co. LLC

套裝中的組件也可單獨購買

產品號碼
描述
SDS
  • C2112Column Preparation SolutionSDS
  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biologySDS
  • R6148RNase A solutionSDS

推薦

產品號碼
描述
訂價

相關產品

產品號碼
描述
訂價

P2308

蛋白酶 K 来源于林伯氏白色念球菌, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology

E1510

溴化乙啶 溶液, BioReagent, for molecular biology, 10 mg/mL in H2O

A9539

琼脂糖, BioReagent, for molecular biology, low EEO

L3287

Escort ™ IV 转染试剂, Lipid reagent for transient and stable transfection of mammalian and insect cells.

T6400

Tris-硼酸-EDTA缓冲溶液, 5× Concentrate

D9780

Lambda DNA Hind III 消化, for DNA electrophoresis

R6148

RNase A 溶液

NA2010

GenElute™ 血液基因组DNA试剂盒, sufficient for 70 purifications

C2112

纯化柱制备液

93221-001

RNAstable®, Tube Kit, for room temp preservation of RNA

T0956

Sigma Universal Transfection Reagent, Polymer reagent for transfecting common mammalian cell lines

SRE0005

蛋白酶 K 来源于林伯氏白色念球菌, Reagents designed and manufactured under current ISO 13485 certification.

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

8A - Combustible corrosive hazardous materials

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

已經擁有該產品？

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Miri Cohen-Zinder et al.
Physiological genomics, 48(5), 367-376 (2016-03-20)
Ecological and economic concerns drive the need to improve feed utilization by domestic animals. Residual feed intake (RFI) is one of the most acceptable measures for feed efficiency (FE). However, phenotyping RFI-related traits is complex and expensive and requires special
S K Sahu et al.
Oncogenesis, 5, e225-e225 (2016-05-10)
Tumor suppressor p53 is a critical player in the fight against cancer as it controls the cell cycle check point, apoptotic pathways and genomic stability. It is known to be the most frequently mutated gene in a wide variety of
Disha Bhange et al.
Frontiers in microbiology, 12, 779472-779472 (2021-12-14)
In a multicentric, observational, investigator-blinded, and longitudinal clinical study of 764 ART-naïve subjects, we identified nine different promoter variant strains of HIV-1 subtype C (HIV-1C) emerging in the Indian population, with some of these variants being reported for the first
Anika Daing et al.
Journal of periodontal & implant science, 42(5), 151-157 (2012-11-28)
Cyclooxygenase (COX) enzyme catalyzes the production of prostaglandins, which are important mediators of tissue destruction in periodontitis. Single nucleotide polymorphisms of COX2 enzyme have been associated with increasing susceptibility to inflammatory diseases. The present study evaluates the association of two
C Castellini et al.
Theriogenology, 78(4), 715-723 (2012-06-19)
There is increasing evidence that the particulate fraction of seminal plasma plays an important role in reproduction of several mammalian species. However, the origin and role of these granules in the physiology of rabbit spermatozoa is partially unknown. The aim
查看所有相關論文

條款

GenElute™ Blood Genomic DNA Kit Protocol

The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

Northern 和 Southern 印迹协议

學習 Northern 和 Southern 印迹的基本知識，以及將大分子轉移到膜支持物上的協議和應用。

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

相關內容

GenElute™ Blood Genomic DNA Kit Protocol

The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務