推薦產品
應用
在分离DNA和RNA的过程中,用于核酸酶的蛋白水解失活。
用于去除内毒素结合的阳离子蛋白,如溶菌酶和核糖核酸酶A。
据报道,其可用于分离肝、酵母和绿豆线粒体
用于测定酶在细胞膜上的定位
用于石蜡包埋组织切片的处理,暴露用于抗体标记的抗原结合位点。
用于可传播海绵状脑病(TSE)研究中朊病毒脑组织样本蛋白的消化。
用于去除内毒素结合的阳离子蛋白,如溶菌酶和核糖核酸酶A。
据报道,其可用于分离肝、酵母和绿豆线粒体
用于测定酶在细胞膜上的定位
用于石蜡包埋组织切片的处理,暴露用于抗体标记的抗原结合位点。
用于可传播海绵状脑病(TSE)研究中朊病毒脑组织样本蛋白的消化。
生化/生理作用
蛋白酶K是一种稳定且具有高活性的丝氨酸蛋白酶。晶体和分子结构研究证据表明该酶属于枯草素家族,具有活性位点催化三联体(Asp39-His69-Ser224)。它在一系列广泛的环境中均稳定:pH,缓冲盐,去污剂(SDS)以及温度。 在0.1-0.5% SDS存在的情况下,蛋白酶K可保持活性,在不损害分离DNA完整性的前提下,可以消化DNA制剂中的多种蛋白质和核酸酶。
單位定義
在pH 7.5,37 °C 条件下,一单位的酶每分钟将会尿素变性的血红蛋白以产生与1.0 μ摩尔 (181 μg) 酪氨酸等同的颜色(Folin-Ciocalteu试剂提供的颜色)。
法律資訊
PicoGreen is a registered trademark of Life Technologies
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建議使用防濺手套
訊號詞
Danger
危險分類
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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文章
蛋白酶 K 可消化結構蛋白、去除核酸酶及分離完整的基因組 DNA,有助於分子生物學應用。
Pro K 有助於破壞細胞膜以釋放 DNA,對下游分子生物學技術至關重要。
Proteinase K aids in molecular biology applications by digesting structural proteins, removing nucleases, and isolating intact genomic DNA.
Pro K aids in disrupting cell membranes for DNA release, crucial for downstream molecular biology techniques.
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