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Merck
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L3287

Sigma-Aldrich

Escort ™ IV 转染试剂

Lipid reagent for transient and stable transfection of mammalian and insect cells.

同義詞:

Gene delivery

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About This Item

分類程式碼代碼:
41106502
NACRES:
NA.25

等級

for molecular biology

品質等級

100

形狀

liquid (aqueous solution)

用途

1 mL sufficient for 160-500 transfections

濃度

1 mg/mL

技術

transfection: suitable

儲存溫度

2-8°C

相關類別

一般說明

Escort™ IV是由专利的聚阳离子脂质体和中性非转染脂质体构成的独特配方。这种脂质体构成的化合物可用于向不同类型的真核细胞转染核酸。

應用

适用于将核酸瞬时和稳定转染到培养的真核细胞中。 每 6 cm 细胞培养板使用约 4-16μL Escort™ IV 和 2μg DNA。方案优化提供了非常高效的转染。关于使用 Escort ™ IV 成功转染的细胞列表，请参见 转染试剂选择指南 。

特點和優勢

  • 适于稳定和瞬时转染
  • 针对广泛的细胞类型进行了优化
  • 低细胞毒性
  • 兼容含血清和不含血清的转染实验
  • 非常适于 Sf9, Sf21 和S2昆虫细胞

成分

Escort™ IV 配方:
1 mg/mL溶于水的总脂质体

请注意，Escort™ IV 中使用的脂质体具体类别属于机密信息。

注意

注意：不要冻存。

原則

在不存在血清条件下，将Escort™ IV与DNA混合，即可形成稳定的复合物。该复合物性质稳定，可直接加入到细胞培养基中，并在培养基中与细胞膜融合，将DNA释放到细胞质中。注意：复合物的形成受到血清抑制，但一旦形成稳定的复合物，即便存在血清也无妨。

法律資訊

Escort is a trademark of Sigma-Aldrich Co. LLC

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves

分析證明 (COA)

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R'ada Massarwa et al.
Developmental cell, 12(4), 557-569 (2007-04-11)
Formation of syncytial muscle fibers involves repeated rounds of cell fusion between growing myotubes and neighboring myoblasts. We have established that Wsp, the Drosophila homolog of the WASp family of microfilament nucleation-promoting factors, is an essential facilitator of myoblast fusion
Victoria J Hammond et al.
The Analyst, 133(1), 71-75 (2007-12-19)
Sol-gel nanoprobes, also known as Photonic Explorer for Bioanalysis with Biologically Localised Embedding (PEBBLE), capable of performing in-vitro intracellular monitoring of reactive oxygen species have been developed using a modified form of 5(6)-carboxyfluorescein diacetate. A sol-gel matrix was selected for
Zev Bryant et al.
Proceedings of the National Academy of Sciences of the United States of America, 104(3), 772-777 (2006-12-22)
Myosin VI supports movement toward the (-) end of actin filaments, despite sharing extensive sequence and structural homology with (+)-end-directed myosins. A class-specific stretch of amino acids inserted between the converter domain and the lever arm was proposed to provide
Melissa D Jordan et al.
Chemical senses, 34(5), 383-394 (2009-03-19)
Moths recognize a wide range of volatile compounds, which they use to locate mates, food sources, and oviposition sites. These compounds are recognized by odorant receptors (OR) located within the dendritic membrane of sensory neurons that extend into the lymph
Manveen K Gupta et al.
Antioxidants & redox signaling, 8(5-6), 1081-1093 (2006-06-15)
Cardiac myocytes, upon exposure to increasing doses of norepinephrine (NE), transit from hypertrophic to apoptotic phenotype. Since reactive oxygen species (ROS) generation is attributed to both phenomena, the authors tested whether an elevation in intracellular ROS level causes such transition.
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文章

細胞轉染簡介

轉染將遺傳物質引入細胞，有助於基因表達和細胞生物學的研究。

Introduction to Cell Transfection

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

條款

Escort™ III Transfection Reagent Protocol

The product bulletin providin detailed use protocol for easy DNA transfection.

Escort™ IV Transfection Reagent Protocol

Product manual provides detailed protocol for easy DNA transfection.

Universal Transfection Reagent Protocol

Universal Transfection Reagent enables efficient nucleic acid delivery into various cells, compatible with different cell culture conditions.

通用轉染試劑協議

通用轉染試劑可將核酸有效地傳送至各種細胞，與不同的細胞培養條件相容。

相關內容

Lipid Transfection Optimization Protocol

Expert guidance on the best way to optimize your transfection experiments. Optimized protocols result in highest efficiency transfections.

Escort™ IV Transfection Reagent Protocol

Product manual provides detailed protocol for easy DNA transfection.

Escort™ III Transfection Reagent Protocol

The product bulletin providin detailed use protocol for easy DNA transfection.

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