跳轉至內容
Merck
全部照片(5)

文件

G1N350

Sigma-Aldrich

GenElute™ 哺乳动物基因组DNA小量制备试剂盒

sufficient for 350 purifications

同義詞:

哺乳动物基因组DNA小量制备, Gen Elute

登入查看組織和合約定價
全部照片(5)

About This Item

分類程式碼代碼:
41105501
NACRES:
NA.55

用途

sufficient for 350 purifications

技術

DNA purification: suitable

儲存溫度

15-25°C

尋找類似的產品？ 前往 產品比較指南

相關類別

一般說明

GenElute哺乳动物基因组DNA纯化试剂盒可以简单、方便地从多种哺乳动物源样本中分离高纯度的大分子量DNA。此类试剂盒使用了为基因组DNA纯化精选的硅胶膜，采用了方便的离心柱形式。

可从培养细胞、组织（包括啮齿动物尾巴）以及新鲜的全血或白血细胞之中分离高纯度的基因组DNA。该试剂盒将硅胶膜技术的优势与微量离心形式结合在一起，同时无需采用昂贵的树脂、乙醇沉淀，以及诸如苯酚和氯仿等有害化学成分。

應用

纯化所得的基因组DNA可直接供以下下游应用使用：
  • 限制性内切酶酶切
  • PCR
  • Southern印迹
  • 测序反应
  • 克隆
  • 连接
GenElute™ Mammalian Genomic DNA Miniprep Kits has been used:
  • to extract DNA from fin samples
  • to extract the BSTUI restriction enzyme from the DNA
  • to isolate DNA from the blood samples extracted from caudal fin
  • to extract tissue and hair follicle DNA

特點和優勢

  • 预期得率：25 μg / 2 x 106个培养细胞；30 μg / 25 mg组织
  • 洗脱体积：200 - 400 μl
  • 所需时间：细胞裂解后20分钟
  • A260/A280比率：1.6 - 1.9
  • 是否需要机械式均质化：否

原則

起始材料首先使用含有离液盐的溶液进行裂解，以确保大分子彻底变性。之后加入乙醇，裂解物离心通过微量离心管时，DNA会结合到硅胶模上。在洗涤去除污染物后，将DNA放在200 μL的Tris-EDTA溶液中洗脱。预期的基因组DNA得率很大程度上取决于所用的起始材料用量及类型。通过本试剂盒纯化所得的DNA的A260/A280比率介于1.6-1.9之间，最大长度可达50 kb。

其他說明

更多信息，请见www.sigma-aldrich.com/genomicdna。

法律資訊

GenElute is a trademark of Sigma-Aldrich Co. LLC

套裝中的組件也可單獨購買

產品號碼
描述
SDS
  • C2112Column Preparation SolutionSDS
  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biologySDS
  • R6148RNase A solutionSDS

推薦

產品號碼
描述
訂價

相關產品

產品號碼
描述
訂價

A9434

氯化铵, for molecular biology, suitable for cell culture, ≥99.5%

P2308

蛋白酶 K 来源于林伯氏白色念球菌, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology

E1510

溴化乙啶 溶液, BioReagent, for molecular biology, 10 mg/mL in H2O

A9539

琼脂糖, BioReagent, for molecular biology, low EEO

G1N70

GenElute™ 哺乳动物基因组DNA小量制备试剂盒, sufficient for 70 purifications

D9281

Lambda DNA EcoR I Hind III Digest, for DNA electrophoresis

L3287

Escort ™ IV 转染试剂, Lipid reagent for transient and stable transfection of mammalian and insect cells.

T6400

Tris-硼酸-EDTA缓冲溶液, 5× Concentrate

G1N10

GenElute™ 哺乳动物基因组DNA小量制备试剂盒, sufficient for 10 purifications

R6148

RNase A 溶液

C2112

纯化柱制备液

93221-001

RNAstable®, Tube Kit, for room temp preservation of RNA

T0956

Sigma Universal Transfection Reagent, Polymer reagent for transfecting common mammalian cell lines

SRE0005

蛋白酶 K 来源于林伯氏白色念球菌, Reagents designed and manufactured under current ISO 13485 certification.

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

8A - Combustible corrosive hazardous materials

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

已經擁有該產品？

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

客戶也查看了

Slide 1 of 3

1 of 3

纯化柱制备液

Sigma-Aldrich

C2112

纯化柱制备液

RNase A 溶液

Sigma-Aldrich

R6148

RNase A 溶液

氯丙嗪 盐酸盐 ≥98% (TLC)

Sigma-Aldrich

C8138

氯丙嗪 盐酸盐

Gudrun Dittrich-Schröder et al.
Molecular ecology resources, 12(1), 109-115 (2011-09-29)
DNA extraction from minute hymenopterans and their larvae is difficult and challenging because of their small size indicating a low amount of starting material. Hence, 11 DNA extraction methods were compared to determine their efficacy in isolating DNA. Success of
Genetic shifts in the transition from wild to farmed white-tailed deer (Odocoileus virginianus) population
Hernandez-Mendoza P M, et al.
International Journal of Biodiversity Science, Ecosystem Services & Management, 10(1), 3-8 (2014)
Inbreeding evidence in a traditional channel catfish (Ictalurus punctatus) hatchery in Mexico
Parra-Bracamonte G M, et al.
Electronic journal of Biotechnology, 14(6), 11-11 (2011)
Michelle N Edelmann et al.
Brain, behavior, and immunity, 25(7), 1299-1304 (2011-03-01)
Variations in maternal care alter the developmental programming of some genes by creating lasting differences in DNA methylation patterns, such as the estrogen receptor alpha (ERα) promoter region. Interestingly, mother rats preferentially lick and groom their male offspring more than
Apollo Simon Peter Balyeidhusa et al.
Veterinary parasitology, 187(1-2), 157-167 (2012-01-17)
The role played by domestic animals in the transmission of gambiense Human African Trypanosomosis remains uncertain. Northwest Uganda is endemic for Trypanosoma brucei gambiense. Of the 3267 blood samples from domestic animals in four counties examined by hematocrit centrifugation technique
查看所有相關論文

條款

GenElute™ 哺乳动物基因组 DNA 微量提取试剂盒实验方案

GenElute 哺乳动物基因组 DNA 纯化方案阐述了从多种哺乳动物来源中分离纯高分子量 DNA 的方法。

GenElute™ Blood Genomic DNA Kit Protocol

The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

GenElute™ Mammalian Genomic DNA Miniprep Kit Protocol

The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

查看全部

相關內容

Mammalian Genomic DNA Miniprep Kit

GenElute Mammalian Genomic DNA Purification Kit isolates pure DNA from mammalian cells using a silica-based membrane in spin columns.

哺乳動物基因組 DNA Miniprep 套件

GenElute 哺乳動物基因組 DNA 純化套件使用旋轉柱中的矽基膜，從哺乳動物細胞中分離出純淨的 DNA。

GenElute™ Blood Genomic DNA Kit Protocol

The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務