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MP0035

Sigma-Aldrich

LookOut ® 支原体 PCR 检测试剂盒

Optimized for use with JumpStart Taq DNA Polymerase, D9307.

同義詞:

PCR-based mycoplasma screening

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

品質

Not suitable for clinical diagnostic use. Will not detect clinically relevant species such as M. pneumoniae and U. urealyticum

包裝

pkg of 1 kit

技術

PCR: suitable

應用

detection
microbiology

相容性

Optimized for use with JumpStart Taq DNA Polymerase, D9307.

儲存溫度

2-8°C

一般說明

该LookOut®支原体PCR检测试剂盒利用聚合酶链反应(PCR),这是因其在对细胞培养基中的支原体无胆甾原体尿素原体以及其他细胞培养衍生的生物制品的检测中具有最高灵敏度而被确定作为选择的方法。该试剂盒已被成功测试具有对20,000至20个拷贝的发酵支原体基因组DNA进行扩增的能力。
包含在试剂盒内的反应管预涂覆有适当的dNTP、引物和上样染料。 相比于需要对反应管进行单独上样的一般方法,整体的检测时间得到了大幅度的下降。

應用

LookOut®支原体PCR检测试剂盒已用于支原体感染筛查。

相容性

该试剂盒已被优化与货号D9307一起使用。不推荐任何带有3′-5′外切核酸酶活性的DNA聚合酶。这些包括Tli、Pfu和Pwo。那些可被使用的DNA聚合酶包括Taq、Tfl和Tth。

法律資訊

JumpStart is a trademark of Sigma-Aldrich Co. LLC
LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany

相關產品

儲存類別代碼

10 - Combustible liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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文章

Detect mycoplasma contamination in cell culture through the PCR, DNA stain, or culture tests. Discover mycoplasma prevention, elimination, and detection kits.

透過 PCR、DNA 染色或培養測試,檢測細胞培養中的支原體污染。探索支原體預防、消除和檢測試劑盒。

回顾使用胎牛(或小牛)血清进行细胞培养的基础知识,以及采购和法规如何影响细胞测定所用血清的价格。

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

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條款

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

細胞培養物的支原體污染是影響細胞模型有效性的嚴重問題。PCR 測試支原體是一種廉價、靈敏且特異的污染檢測方法。

相關內容

探索我们广泛的胎牛血清(FBS)产品组合并根据您的实验室需求和预算选择合适的产品。了解全球事件如何影响FBS价格。

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

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