The product MAK106 uses linear regression fitting. The standard curve is prepared based on the donor particle concentration indicated on the label, which is batch-specific. The standard curve in table 2 is representative and for example purposes only. It's important to note that the concentrations may vary. The pmole values are calculated through dimension analysis using the amount of donor particle solution used and any subsequent dilution. For example, the most concentrated standard can be calculated by considering the volume of the donor particle. In this case, the most concentrated standard has 130 pmoles. Each subsequent dilution is two times less concentrated, resulting in concentrations of 65, 32.5, 16.25, and 8.1 pmoles.
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用途
sufficient for 100 fluorometric tests
應用
pharmaceutical
檢測方法
fluorometric
相關疾病
cardiovascular diseases
儲存溫度
2-8°C
基因資訊
human ... CETP(1071)
一般說明
Cholesteryl ester transfer protein (CETP) is present in normal human plasma. The protein transfers neutral lipids from high density lipoproteins (HDL) to very low density lipoprotein (VLDL) and low density lipoprotein (LDL). CETP plays an important role in lipoprotein metabolism and influences the reverse cholesterol transport pathway. The method is useful for measuring CETP activity in plasma or serum in all species that express CETP.
應用
Cholesteryl ester transfer protein (CETP) activity Assay Kit has been used for measurement of CETP activity in the plasma samples.[1]
特點和優勢
Compatible with high-throughput handling systems.
適合性
Suitable for the detection of cholesteryl ester transfer protein (CETP) in plasma / serum (fresh or frozen), recombinant or purified CETP.
原則
The CETP Activity Assay Kit uses a proprietary substrate that enables the detection of CETP-mediated transfer of neutral lipid from the substrate to a physiological acceptor. The transfer activity results in an increase in fluorescence intensity (λEx=535 nm/λEm=587 nm). In a total volume of 200 μL, the assay is linear from 0.2 to 0.8 μL of normal human plasma. Assay results not affected by endogenous plasma HDL, LDL or VLDL concentrations.
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描述
訂價
儲存類別代碼
10 - Combustible liquids
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相關內容
The CETP Activity Assay Kit enables the detection of CETP-mediated transfer of neutral lipids.
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What type of regression fitting is used for the product MAK106, and what is the method for calculating the pmoles shown in the typical standard curve in table 2?
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