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CS1090

Sigma-Aldrich

HMG-CoA 还原酶检测试剂盒

sufficient for 30 assays (1 mL), sufficient for 100 assays (200 μL)

同義詞:

HMGR 检测试剂盒

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.28
暫時無法取得訂價和供貨情況

用途

sufficient for 100 assays (200 μL)
 sufficient for 30 assays (1 mL)

品質等級

200

運輸包裝

dry ice

儲存溫度

−70°C

基因資訊

human ... HMGCR(3156)

相關類別

一般說明

HMG-CoA 还原酶测定试剂盒是胆固醇和其它相关代谢途径基础研究的重要工具。

應用

HMGR活性试剂盒可用于测定HMG-CoA还原酶活性。该试剂盒的主要功能是筛选HMG-CoA还原酶的纯化后催化亚基的不同抑制因子和激活因子，这在临床治疗中具有重要意义。该检测方法针对340nm处的吸光度下降进行光谱测量，而后者恰恰反映了存在HMG-CoA底物时HMGR催化亚基对NADPH的氧化水平。

生化／生理作用

HMGR（3-羟基-3-甲基戊二酰-CoA还原酶）是位于内质网上的一种跨膜糖蛋白。[1] 这种酶可以催化HMG-CoA的四电子还原反应，生成辅酶A（CoA）和甲羟戊酸。这是固醇生物合成中的限速步骤。[2] 为了维持衍生的甲基戊酸酯的浓度，可以通过合成、降解、磷酸化等方式控制HMGR的活性。除了HMGR的生理调控之外，人的HMGR酶可成功通过药物来靶定调控，可用于高血清胆固醇的临床治疗。[3][4] 控制血清胆固醇体水平具有非常重要的治疗作用，这是因为血胆脂醇过多往往会引起动脉硬化病症加剧，进而引起心血管疾病，这可能会引起冠心病和中风。目前已有证据表明，胆固醇体内平衡受到干扰会引起慢性炎症恶化。[5]

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產品號碼
描述
SDS
  • N6505β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate, ≥95% (HPLC) 25 mgSDS

儲存類別代碼

11 - Combustible Solids

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
0000415146
0000415146
0000364958
0000364958
0000261885

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A J Koning et al.
Molecular biology of the cell, 7(5), 769-789 (1996-05-01)
In all eucaryotic cell types analyzed, proliferations of the endoplasmic reticulum (ER) can be induced by increasing the levels of certain integral ER proteins. One of the best characterized of these proteins is HMG-CoA reductase, which catalyzes the rate-limiting step
Matteo Mozzicafreddo et al.
Journal of lipid research, 51(8), 2460-2463 (2010-04-27)
Radioisotope-based and mass spectrometry coupled to chromatographic techniques are the conventional methods for monitoring HMG-CoA reductase (HMGR) activity. Irrespective of offering adequate sensitivity, these methods are often cumbersome and time-consuming, requiring the handling of radiolabeled chemicals or elaborate ad-hoc derivatizing
R Kleemann et al.
Current drug targets. Cardiovascular & haematological disorders, 5(6), 441-453 (2006-03-01)
Besides classical risk factors such as hypercholesterolemia and hypertension, chronic subacute inflammation has recently been recognized as an important force driving the development of atherosclerosis, the most common underlying cause of myocardial infarction and stroke. There is compelling evidence that
G A Holdgate et al.
Biochemical Society transactions, 31(Pt 3), 528-531 (2003-05-30)
The statins are inhibitors of 3-hydroxy-3-methylglutaryl CoA (HMG-CoA) reductase (HMG-CoAR), and are utilized to decrease levels of atherogenic lipoproteins in patients with, or who are at high risk of, cardiovascular disease. This study describes the inhibition of a recombinant, catalytic
Huanjie Li et al.
Frontiers in cell and developmental biology, 8, 404-404 (2020-07-17)
Statins are widely used cholesterol-lowering drugs. Their potential application in anti-cancer treatment is also under investigation. The individual variance in statin response has been observed, which may be caused by the variation in human HMG-CoA reductase (hHMGR)-the inhibition target of
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文章

HMG-CoA Reductase (HMGR) Assay Kit

3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) is a transmembrane glycoprotein, located on the endoplasmic reticulum.

Questions

1–10 of 10 Questions  

  1. How many samples can we analyse using 1Kt?

    1 answer

    1. Tables 1 and 2 provide specific instructions for sample preparation for reaction volumes in a cuvette (1 mL) or a 96 well plate. The limiting reagent based upon the supplied kit components is the HMG-COA. There is 2 mL of HMG-COA supplied and 0.18 mL used in total for the blank, activity and inhibitor well preparations. This means that approximately 33 wells or 30 assays can be performed as indicated in the kit bulletin.

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/240/783/cs1090pis-ms.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  4. What is the concentration of the HMG CoA substrate (S7447)?

    1 answer

    1. The concentration of the HMG CoA substrate, product number S7447, is 0.5% in water.

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  5. Is this suitable for the analysis of compounds dissolved in DMSO for comparing the ability of some compounds to inhibit HMGCR? Specifically, using 1 ul of the compound solution (0.05, 0.5, 5 mM stock buffer) and 1 ul DMSO (solvent alone) as control, the signal @ 340 nM did not significantly decrease after a 10-minute kinetic program (signal capture every 20 seconds according to the protocol). There was only a 15-20 percent decrease in the read. Is this normal, or is DMSO incompatible with the enzyme reaction, or can some improvement be made?

    1 answer

    1. As per the QC specialist group, in the product CS1090, there isn't any experience with DMSO (all kit's components are without DMSO), so the information cannot provided about DMSO incompatibility. After reviewing the raw data, a good slope was observed with the enzyme; for example, with 6ug (10ul) enzyme, the slope was 0.3-0.5.

      Helpful?

  6. •What is the concentration of the HMG-CoA (Cat.No. S7447)? and What solvent is it dissolved in?

    1 answer

    1. The concentration of HMG-CoA is the composition of 99.5 % water and 0.5 % (DL-3-Hydroxy-3-methylglutaryl) coenzyme aso-salt trihydrate.

      Helpful?

  7. Product CS1090, kit component of HMG-CoA Reductase Assay Kit Product I5909, Pravastatin, is the concentration 100uM correct?

    1 answer

    1. Yes, the concentration for Pravastatin in this kit is 100 micromolar (100 μM)

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  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  9. What is the lowest concentration that you can use Pravastatin and observe inhibition in Product CS1090, HMG-CoA Reductase Assay kit?

    1 answer

    1. For Product CS1090, HMG-CoA Reductase Assay kit we do not observe any inhibition when using Pravastatin at 25 or 50 nM.  We observe inhibition of HMG-CoA Reductase with 250 nM pravastatin.

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  10. For Product CS1090, HMG-CoA Reductase Assay Kit, what is the concentration of kit component Product I5909, Pravastatin?

    1 answer

    1. The concentration of Pravastatin, Product I5909 in Product CS1090, HMG-CoA Reductase Assay Kit,  is 100 uM solution in assay buffer.

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