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L3908

Sigma-Aldrich

β-乳球蛋白 来源于牛奶

≥90% (PAGE), lyophilized powder

同義詞:

βLg, BLG, Bos d 5, beta-lactoglobulin

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About This Item

CAS號碼:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.61
暫時無法取得訂價和供貨情況

生物源

bovine milk

品質等級

化驗

≥90% (PAGE)

形狀

lyophilized powder

技術

ELISA: suitable

UniProt登錄號

儲存溫度

2-8°C

基因資訊

bovine ... LGB(280838)

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一般說明

来自奶牛的牛奶含有β-乳球蛋白(BLG)。它天然存在许多遗传变异体,最普遍的牛变异体是BLG A和BLG B。[1]

應用

β-乳球蛋白被用于细胞学检测,以诊断肠易激综合征患者的食物超敏反应。[2]

生化/生理作用

βLg是脂质运载蛋白家族的成员,它是由162个氨基酸组成的小分子蛋白,分子量约为18,400Da。它具有由八条链组成的β桶状结构(A-H链),桶外存在一个具有三个转角的a螺旋和第九条β链(I链),其形成二聚体形成界面的一部分。[3]

其他說明

含 β-乳球蛋白 A 和 B,可通过色谱法分离。

品質

可能不含叶酸结合蛋白;不推荐用于叶酸分析。

準備報告

色谱纯化产品

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

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Sirpa Jylhä et al.
Journal of immunological methods, 350(1-2), 63-70 (2009-08-04)
Cow's milk allergy (CMA) is a common food allergy, especially among infants and young children. Approximately 85% of milk-allergic children outgrow their allergy by the age of three but the remaining 15% remain allergic. Bovine beta-lactoglobulin (BLG) is one of
Lena Wartosch et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 23(12), 4056-4068 (2009-08-08)
Mutations in either ClC-7, a late endosomal/lysosomal member of the CLC family of chloride channels and transporters, or in its beta-subunit Ostm1 cause osteopetrosis and lysosomal storage disease in mice and humans. The severe phenotype of mice globally deleted for
Tilman Barz et al.
Journal of chromatography. A, 1217(26), 4267-4277 (2010-05-07)
In this work, parameters of the steric mass-formalism SMA are optimally ascertained for a reliable determination of the adsorption isotherms of beta-lactoglobulin A and B under non-isocratic conditions. For this purpose, static batch experiments are used in contrast to the
Jonathan Vaneyck et al.
The Journal of biological chemistry, 296, 100358-100358 (2021-02-05)
The aggregation of the protein α-synuclein (aSyn) into amyloid fibrils in the human brain is associated with the development of several neurodegenerative diseases, including Parkinson's disease. The previously observed prion-like spreading of aSyn aggregation throughout the brain and the finding
Junzhen Zhong et al.
Food chemistry, 278, 491-496 (2018-12-26)
Previous work indicated that conformational changes of β-lactoglobulin (β-LG) induced by dynamic high pressure microfluidization (DHPM) was related to the increase of antigenicity. In this study, β-LG glycated with 1-kestose and combined with DHPM decreased the antigenicity of β-LG. The

Questions

1–6 of 6 Questions  
  1. What is the preferred buffer to dilute the product in? Or is it deionized water with or without protease inhibitors? (Application: SDS-PAGE gels and western blot)

    1 answer
    1. This product is not tested for solubility. Various sources suggest that this material may be reconstituted in water or aqueous buffers at 5 mg/ml. This information has not been validated.

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  2. does this product have other components such as sucrose, trehalose or salts?

    1 answer
    1. This product is prepared from skimmed milk. Lactoglobulins are precipitated and further purified chromatographically. The final dialysis process depletes most residual salts. The minimum purity of this product is 90%. While sugars such as trehalose and sucrose would not be expected, the impurities of this product have not been identified.

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  3. How is the solubility of the product in water?

    1 answer
    1. This product is not tested for solubility. Various sources suggest that this material may be reconstituted in water or aqueous buffers at 5 mg/ml. This information has not been validated.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  5. What is the molecular weight of Product L3908, β-Lactoglobulin from bovine milk?

    1 answer
    1. Product L3908, β-Lactoglobulin from bovine milk, exists as a dimer of two peptides. Peptide A = 18,367, B = 18,281; the monomer is approximately 36,600.

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  6. How does the storage temperature relate to shipping conditions?

    1 answer
    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

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